J Korean Thyroid Assoc.  2012 May;5(1):39-44. 10.11106/jkta.2012.5.1.39.

Alpha-Lipoic Acid Decreases Iodine Uptake in the Rat Thyroid Cell Line FRTL-5

Affiliations
  • 1Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. tykim@amc.seoul.kr
  • 2Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Abstract

BACKGROUND AND OBJECTIVES
Alpha-lipoic acid (ALA) is a naturally occurring fatty acid with strong antioxidant properties that exerts protective effects against harmful free radical damage. The aim of the present study was to assess the effect of ALA on iodine uptake and expression of sodium iodine symporter (NIS) using FRTL-5 cells.
MATERIALS AND METHODS
Cells were treated with ALA for various time and doses, in the presence or absence of thyrotropin (TSH). Cell viability assay, iodine uptake assay and NIS promoter activity assay were performed.
RESULTS
ALA increased NIS promoter activity. It showed an additive effect when concomitantly added with TSH. After 48 hours of incubation with ALA in the presence or absence of TSH, there was no difference in iodine uptake according to doses of ALA. After 72 hours of incubation with ALA and TSH, ALA decreased iodine uptake in dose-dependent way.
CONCLUSION
ALA induced NIS gene transcription of FRTL-5, but suppressed iodine uptake in the presence of TSH. ALA may suppress iodine uptake through effect for post-translation stage of NIS protein.

Keyword

Thyroid; Alpha-lipoic acid; Sodium iodine symporter

MeSH Terms

Animals
Cell Line
Cell Survival
Iodine
Ion Transport
Rats
Sodium
Symporters
Thioctic Acid
Thyroid Gland
Thyrotropin
Iodine
Sodium
Symporters
Thioctic Acid
Thyrotropin
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