J Korean Neurosurg Soc.  2015 Aug;58(2):101-106. 10.3340/jkns.2015.58.2.101.

T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects

Affiliations
  • 1Department of Hand & Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, China. LiangfuJiangcn@163.com
  • 2Department of Orthopaedics, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Zhejiang, China.

Abstract


OBJECTIVE
The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects.
METHODS
ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the following methods : Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft.
RESULTS
At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of CD3+, CD4+, and CD8+ subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-alpha, and IFN-gamma in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-alpha, and IFN-gamma were significantly reduced in group C (p<0.05).
CONCLUSION
The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects.

Keyword

Neural transplantation; Immunity; T cell subsets; Cytokines

MeSH Terms

Allografts
Animals
Autografts
Cytokines*
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Interferon-gamma
Interleukin-2
Mesenchymal Stromal Cells*
Orbit
Rats*
Rats, Sprague-Dawley
Rats, Wistar
Sciatic Nerve
T-Lymphocyte Subsets*
Transplants
Tumor Necrosis Factor-alpha
Cytokines
Interferon-gamma
Interleukin-2
Tumor Necrosis Factor-alpha

Figure

  • Fig. 1 Repairing sciatic nerve defects with artificial nerve.

  • Fig. 2 The expression of CD44 (A), CD45 (B), and CD90 (C) of ADSCs determined using flow cytometry. ADSCs : adipose-derived mesenchymal stem cells.

  • Fig. 3 Longitudinal section of acellular nerve displayed an intact three-dimensional structure (scanning electron microscopy, 5000×). IL-2 : interleukin-2, TNF-α : tumor necrosis factor-α, IFN-γ : interferon-γ.

  • Fig. 4 The proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, IFN-γ, and TNF-α. IL-2 : interleukin-2, TNF-α : tumor necrosis factor-α, IFN-γ : interferon-γ.


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