Cancer Res Treat.
2004 Aug;36(4):263-270.
Detection of RTP801, a Gene That is Differentially Expressed in Irradiated HeLa Cells
- Affiliations
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- 1Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea.
- 2Department of Radiation Oncology, College of Medicine, Chungnam National University, Daejeon, Korea. mjcho@cnu.ac.kr
- 3Department of Biology, College of Natural Sciences, Chungnam National University, Daejeon, Korea.
- 4Cancer Research Institute, Chungnam National University, Daejeon, Korea.
Abstract
- PURPOSE
To quantify the effects of irradiation on the expression levels of a differentially expressed gene, RTP801, in HeLa cells. MATERIALS AND METHODS: Total RNA was isolated from irradiated and non-irradiated HeLa cells. A subtraction library was constructed, from which 88 random clones were screened. The expression patterns of one clone, detected by reverse Northern blotting, were quantified by real time RT-PCR, using CYBR green I dye. RESULTS: RTP801, a hypoxia-inducible factor-I-responsive gene, was identified as a differentially expressed gene in HeLa cells exposed to X-ray. Real time RT-PCR showed that the mRNA levels of RTP801 were greatly diminished by radiation. CONCLUSIONS: These results suggest that down-regulation of hypoxia-inducible factor-I-responsive genes, such as RTP801, in irradiated HeLa cells may result in reductions in the radiotherapy resistance of tumor cells.
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