Ann Dermatol.  2011 May;23(2):150-155. 10.5021/ad.2011.23.2.150.

Effects of Human Adipose-derived Stem Cells on Cutaneous Wound Healing in Nude Mice

• Lee SH
• Lee JH
• Cho KH

• KMID: 2156655
• DOI: http://doi.org/10.5021/ad.2011.23.2.150

Abstract

BACKGROUND
Despite numerous treatments available for deteriorated cutaneous wound healing such as a diabetic foot, there is still the need for more effective therapy. Adipose-derived stem cells (ASCs) are mesenchymal stem cells, which are self-renewing and multipotent. Mesenchymal stem cells have the potential for tissue repair and regeneration.
OBJECTIVE
To investigate the effects of human ASCs on the healing of cutaneous wounds in nude mice.
METHODS
15-mm round full-thickness skin defects were generated on the back of BALB/c nude mice. The mice were divided into three groups for wound coverage: (i) human ASCs-populated collagen gel, (ii) human dermal fibroblasts-populated collagen gel, and (iii) collagen gel alone. Wound contraction was prevented with a splint method. Wound size was measured 10 days after injury. At 28 days histological analysis was performed.
RESULTS
Both ASCs and dermal fibroblasts accelerated wound closure, but dermal fibroblasts were more effective than ASCs. At 28 days, the dermal portion of ASCs or dermal fibroblasts wound scars were thicker than collagen gel wound scars.
CONCLUSION
ASCs and dermal fibroblasts stimulate cutaneous wound healing and improve scar thickness.

Keyword

Adipose tissue; Fibroblast; Mesenchymal stem cell; Mouse; Wound healing

MeSH Terms

Adipose Tissue
Animals
Cicatrix
Collagen
Contracts
Diabetic Foot
Fibroblasts
Humans
Mesenchymal Stromal Cells
Mice
Mice, Nude
Skin
Splints
Stem Cells
Wound Healing
Collagen