Immune Netw.  2007 Dec;7(4):197-202. 10.4110/in.2007.7.4.197.

Lectins Isolated from Mushroom Fomitella fraxinea Enhance MHC-restricted Exogenous Antigen Presentation

• Kim H
• Cho KM
• Gerelchuluun T
• Lee JS
• Chung KS
• Lee CK

• KMID: 2150637
• DOI: http://doi.org/10.4110/in.2007.7.4.197

Abstract

BACKGROUND: Immunomodulators enhancing MHC-restricted antigen presentation would affect many cellular immune reactions mediated by T cells or T cell products. However, modulation of MHC-restricted antigen presentation has received little attention as a target for therapeutic immunoregulation. Here, we report that lectins isolated from mushroom Fomitella fraxinea enhance MHC-restricted exogenous antigen presentation in professional antigen presenting cells (APCs).
METHODS
Lectins, termed FFrL, were isolated from the carpophores of Fomitella fraxinea, and its effects on the class I and class II MHC-restricted presentation of exogenous ovalbumin (OVA) were examined in mouse dendritic cells (DCs) and mouse peritoneal macrophages. The effects of FFrL on the expression of total MHC molecules and the phagocytic activity were also examined in mouse DCs.
RESULTS
DCs cultured in the presence of FFrL overnight exhibited enhanced capacity in presenting exogenous OVA in association with class I and class II MHC molecules. FFrL increased slightly the total expression levels of both class I (H-2K(b)) and class II (I-A(b)) MHC molecules and the phagocytic activity of DCs. Antigen presentation-enhancing activity of FFrL was also observed in macrophages isolated from mouse peritoneum.
CONCLUSION
Lectins isolated from the carpophores of Fomitella fraxinea increase MHC-restricted exogenous antigen presentation by enhancing intracellular processing events of phagocytosed antigens.

Keyword

Fomitella fraxinea; lectin; MHC-restricted antigen presentation; dendritic cell; macrophage

MeSH Terms

Agaricales*
Animals
Antigen Presentation*
Antigen-Presenting Cells
Dendritic Cells
Immunologic Factors
Lectins*
Macrophages
Macrophages, Peritoneal
Mice
Ovalbumin
Ovum
Peritoneum
T-Lymphocytes
Immunologic Factors
Lectins
Ovalbumin