Korean J Otolaryngol-Head Neck Surg.
1999 Jan;42(1):72-81.
Establishment and Characterization of a Novel Cell Line (PNUH-12) Derived from a Human Squamous Cell Carcinoma of the Hypopharynx
- Affiliations
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- 1Department of Otorhinolaryngology, College of Medicine, Pusan National University, Pusan, Korea. rohhj@hyowon.cc.pusan.ac.kr
- 2Medical Research Institute of Pusan National University Hospital, Pusan, Korea.
- 3Department of Clinical Pathology, College of Medicine, Dong-A University, Pusan, Korea.
Abstract
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BACKGROUND AND OBJECTIVES: Cancer cell line is the basic material of various cancer research. Diverse cancer cell lines from various head and neck regions are needed for biologic research of head and neck cancer. However, cell lines derived from head and neck cancer are not common. Recently, we have established and characterized a novel human squamous carcinoma cell line, PNUH-12, from the hypopharynx.
MATERIALS AND METHODS
Among trials of twenty cases of head and neck cancer, we established only one specimen succeeded culture passage over 50. We characterized the cell line as follows: growth pattern and curve, morphology using phase contrast microscope and transmission electromicroscope, chromosomal anaysis, flow cytometric analysis, tumorigenecity by xenograft of cell line into nude mouse and morphological comparison, expression of cytokeratin, epithelial membrane antigen and vimentin, and P 53 mutation and its sequencing.
RESULTS
PNUH-12 showed typical growth pattern of cancer cell line, representative morphological characteristics of squamous epithelial cell origin, multiple numerical and structural clonal abnormalities of chromosome, aneuploidy pattern of flow cytometry, and strong expression of cytokeratin. The formed tumor of nude mouse showed the identical histopathological phenotype (squamous cell carcinoma) of the original tumor of patient and similar morphology of PNUH-12. There was one point mutation of 78th base, C to G, in exon 7 of P53 gene.
CONCLUSION
PNUH-12 can be a good control material and successufully bestowed to researchers for study of biology in head and neck cancer. There still needed more head and neck cancer cell lines from various regions and diverse cell types in future.
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