Korean J Anat.
2005 Feb;38(1):11-19.
Cytoplasmic Domain of Intercellular Adhesion Molecule-1(ICAM-1)is Important for the ICAM-1 Mediated Membrane Projection of Endothelial Cells
- Affiliations
-
- 1Digestive Disease Research University, Wonkwang University School of Medicine, Korea.
- 2Department of Anatomy, Wonkwang University School of Medicine, Korea.
- 3Department of Microbiology and Immunology, Wonkwang University School of Medicine, Korea. cdjun@wonkwang.ac.kr
- 4Medicinal Resources Research Institute, Iksan, Chonbuk 570-749, Korea.
- 5Department of Molecular Biology, Chonbuk National University, Chonju, Chonbuk 561-756, Korea.
Abstract
- Intercellular adhesion molecule-1 (ICAM-1)has been shown to enhance leukocyte adhesion, thereby inducing migration through blood endothelial cells. However, the molecular event during the process of adhesion is largely unknown. To examine the role of ICAM-1 cytoplasmic domain in SDF-1 alpha-induced T lymphocyte migration and adhesion, mutant human ICAM-1 molecules were expressed in COS-7 cell line. COS-7 cells expressing ICAM-1_GFP mutant without alpha-actinin revealed no association with the actin cytoskeleton, while wild-type ICAM-showed clear association with the actin, as observed by confocal microscopy, suggesting that actinin binding motif in the cytoplasmic domain of ICAM-1 is important for the proper localization of ICAM-1 on the cell membrane. However, based on adhesion assay, we found that the cytoplasmic domain of ICAM-1 is not essential for the binding of lymphocytes which were activated by SDF-1alpha. On the other hand, ICAM-1-mediated receptor-ligand clustering event was significantly inhibited in the cells expressing ICAM-1 mutants without alpha-actinin or whole cytoplasmic domain. Taken together, these results suggest that ICAM-1 cytoplasmic domain is not essential for the adhesion but important for the ligand-receptor-mediated membrane projection of endothelial cells before trans-endothelial migration of lymphocytes.
Keyword
MeSH Terms
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Actin Cytoskeleton
Actinin
Actins
Animals
Cell Membrane
Chemokine CXCL12
COS Cells
Cytoplasm*
Endothelial Cells*
Hand
Humans
Intercellular Adhesion Molecule-1*
Leukocytes
Lymphocyte Function-Associated Antigen-1
Lymphocytes
Membranes*
Microscopy, Confocal
Actinin
Actins
Chemokine CXCL12
Intercellular Adhesion Molecule-1
Lymphocyte Function-Associated Antigen-1
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