J Breast Cancer.  2006 Mar;9(1):14-17. 10.4048/jbc.2006.9.1.14.

Mutational Analysis of 17beta-hydroxysteroid dehydrogenase type 2 gene in Breast Cancers

• Cho MS
• Kim HA
• Lee SN

• KMID: 1976162
• DOI: http://doi.org/10.4048/jbc.2006.9.1.14

Abstract

PURPOSE
: The 17beta-hydroxysteroid dehydrogenases(17HSDs) play an important role in the regulation of the physiologic activities of sex steroid hormones. The predominance of 17HSD type 1 in the malignant breast tissue could increase the estrogen-dependent proliferation and stimulate the cancer progression. On the other hand, the oxidative 17HSD type 2 may protect the normal breast cells from an excessive estradiol effect. To identify the role of 17HSD type 2 in the carcinogenesis of breast cancer, we investigate the mutation of 17HSD type 2 in 35 breast cancers.
METHODS
: We analyzed the entire coding region of the 17 HSD type 2 gene for detection of the somatic mutations in 35 invasive ductal carcinomas of the breast by polymerase chain reaction, single strand conformation polymorphism, and DNA sequencing.
RESULTS
: We found one missense mutation in exon 6(2.86%). It revealed the CCT-->CTT (Pro-->Leu) transition type at codon 262 in exon 6.
CONCLUSION
: In present study, we found only a mutation of the 17beta -HSD type 2 gene in breast cancer and could not demonstrate the direct relationship between the mutation of the 17beta -HSD type 2 gene and the development of breast cancer. These results suggest that the mutation of 17HSD type 2 doesn? play a major role in the development of breast cancer.

Keyword

Breast cancer; 17HSD type 2

MeSH Terms

Breast Neoplasms
Breast*
Carcinogenesis
Carcinoma, Ductal
Clinical Coding
Codon
Estradiol
Exons
Gonadal Steroid Hormones
Hand
Mutation, Missense
Oxidoreductases*
Polymerase Chain Reaction
Sequence Analysis, DNA
Codon
Estradiol
Gonadal Steroid Hormones
Oxidoreductases

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