Korean J Parasitol.
2013 Oct;51(5):573-577.
Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR
- Affiliations
-
- 1State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. wangyh061001@163.com
- 2Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. zhangdl2005@163.com
- 3Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. yinhong@caas.net.cn
Abstract
- A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.
MeSH Terms
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Animals
Azure Stains
Biological Assay
Brain/parasitology
DNA, Protozoan/*blood/genetics
Lung/parasitology
Mice
Nucleic Acid Amplification Techniques/*veterinary
Parasitemia
Real-Time Polymerase Chain Reaction/veterinary
Sensitivity and Specificity
Swine
Swine Diseases/*diagnosis/parasitology
Toxoplasma/genetics/*isolation & purification
Toxoplasmosis, Animal/*diagnosis/parasitology
Azure Stains
DNA, Protozoan
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