Korean J Vet Res.
2014 Jun;54(2):113-115.
Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces
- Affiliations
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- 1Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang 430-757, Korea. jaewon8911@korea.kr
Abstract
- Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were 10(4), 10(5) and 10(2) CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.
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