Korean J Vet Res.  2014 Jun;54(2):113-115.

Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces

Affiliations
  • 1Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Anyang 430-757, Korea. jaewon8911@korea.kr

Abstract

Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella infection. The detection limits for broth dilution, spiked feces and enrichment were 10(4), 10(5) and 10(2) CFUs/mL, respectively. The LAMP assay developed in the present study may be a reliable method for detection of Salmonella spp. in pig feces.

Keyword

detection limit; invA; loop-mediated isothermal amplification assay; Salmonella

MeSH Terms

Animals
Diagnosis
Feces*
Gastroenteritis
Limit of Detection
Salmonella Infections
Salmonella*
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