Korean J Vet Res.  2013 Dec;53(4):217-224.

Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice

Affiliations
  • 1Plant System Engineering Center, KRIBB, 125 Gwahangno, Yuseong-gu, Daejeon 305-806, Korea. hyuns@kribb.re.kr
  • 2Microbial Resource Center, KRIBB, 125 Gwahangno, Yuseong-gu, Daejeon 305-806, Korea.
  • 3Laboratory Animal Resource Center, KRIBB, 685-1, Ochang 305-333, Korea.
  • 4National Institute of Animal Science, RDA, Cheonan 330-801, Korea.
  • 5School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea.

Abstract

Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-S0.7) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-S0.7 gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-S0.7 protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-S0.7 antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses.

Keyword

oral administration; potato-derived antigen; transmissible gastroenteritis virus; transgenic plant

MeSH Terms

Administration, Oral
Animals
Blotting, Northern
Blotting, Western
Coronavirus
DNA
Enzyme-Linked Immunosorbent Assay
Escherichia
Gastroenteritis
Glycoproteins*
Immunoglobulin A
Immunoglobulins
Mice*
Plants, Genetically Modified
Polymerase Chain Reaction
RNA, Messenger
Solanum tuberosum*
Transmissible gastroenteritis virus*
Vaccines
DNA
Glycoproteins
Immunoglobulin A
Immunoglobulins
RNA, Messenger
Vaccines
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