J Korean Med Sci.  2005 Oct;20(5):759-763. 10.3346/jkms.2005.20.5.759.

Additive Effect of Diesel Exhaust Particulates and Ozone on Airway Hyperresponsiveness and Inflammation in a Mouse Model of Asthma

• Jang AS
• Choi IS
• Takizawa H
• Rhim TY
• Lee JH
• Park SW
• Park CS

• KMID: 1781757
• DOI: http://doi.org/10.3346/jkms.2005.20.5.759

Abstract

Allergic airway diseases are related to exposure to atmospheric pollutants, which have been suggested to be one factor in the increasing prevalence of asthma. Little is known about the effect of ozone and diesel exhaust particulates (DEP) on the development or aggravation of asthma. We have used a mouse asthma model to determine the effect of ozone and DEP on airway hyperresponsiveness and inflammation. Methacholine enhanced pause (P(enh)) was measured. Levels of IL-4 and IFN-gamma were quantified in bronchoalveolar lavage fluids by enzyme immunoassays. The OVA-sensitized-challenged and ozone and DEP exposure group had higher P(enh) than the OVA-sensitized-challenged group and the OVA-sensitized-challenged and DEP exposure group, and the OVA-sensitized-challenged and ozone exposure group. Levels of IFN-gamma were decreased in the OVA-sensitized-challenged and DEP exposure group and the OVA-sensitized-challenged and ozone and DEP exposure group compared to the OVA-sensitized-challenged and ozone exposure group. Levels of IL-4 were increased in the OVA-sensitized-challenged and ozone exposure group and the OVA-sensitized-challenged and DEP exposure group, and the OVA-sensitized-challenged and ozone and DEP exposure group compared to OVA-sensitized-challenged group. Co-exposure of ozone and DEP has additive effect on airway hyperresponsiveness by modulation of IL-4 and IFN-gamma suggesting that DEP amplify Th2 immune response.

Keyword

Vehicle Emissions; Air Pollutants; Diesel Exhaust Particulates; Ozone; Bronchial Hyperreactivity

MeSH Terms

Air Pollutants, Environmental/toxicity
Animals
Asthma/*chemically induced/*immunology
Disease Models, Animal
Drug Combinations
Drug Synergism
Female
Hypersensitivity/complications/*etiology/*immunology
Interferon Type II/immunology
Interleukin-4/immunology
Mice
Mice, Inbred BALB C
Ovalbumin
Ozone/*toxicity
Pneumonia/*chemically induced/complications/*immunology
Research Support, Non-U.S. Gov't
Respiratory Hypersensitivity/chemically induced/complications/immunology
Vehicle Emissions/*toxicity