Korean J Physiol Pharmacol.  2006 Dec;10(6):343-347.

Upregulation of Renin-angiotensin, Endothelin and C-type Natriuretic Peptide in Rat Glomerulus with Bilateral Ureteral Obstruction

• Bae EH
• Kim SW

Abstract

The present study was designed to investigate the effects renin-angiotensin-aldosterone system (RAAS), endothelin (ET) and local natriuretic peptide (NP) system for glomerulopathy induced in the experimental bilateral ureteral obstructive rats. Sprague-Dawley male rats (200~220 g body weight) were bilaterally obstructed by ligation of the proximal ureters for 24 hours. Control rats were treated in the same ways, except that no ligature was made. The glomeruli were isolated from cortex by graded sieve methods, and the mRNA expressions of local renin-angiotensin system (RAS), aldosterone synthase (CYP11B2), endothelin-1 (ET-1) and NP system were determined by real-time polymerase chain reaction. Following the bilateral ureteral obstruction, the mRNA expressions of renin, angiotensin converting enzyme 1 as well as ET-1 were increased, while that of angiotensin converting enzyme 2 was not changed. The expressions of CYP11B2 and angiotensin II receptors were not changed. C-type natriuretic peptide (CNP) expression was increased, while its receptors (natriuretic peptide receptor-B) were not changed. We suggest that the upregulation of local RAS and ET play a role in the progressive glomerular injury, and that the enhanced CNP activity also plays a compensatory role in obstructive uropathy in the glomerulus.

Keyword

Bilateral ureteral obstruction; Renin-angiotensin system; Aldosterone synthase; C-type natriuretic peptide; Endothelin; Glomerulus

MeSH Terms

Aldosterone Synthase
Animals
Endothelin-1
Endothelins*
Humans
Ligation
Male
Natriuretic Peptide, C-Type*
Peptidyl-Dipeptidase A
Rats*
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Receptors, Angiotensin
Renin
Renin-Angiotensin System
RNA, Messenger
Up-Regulation*
Ureter*
Ureteral Obstruction*
Aldosterone Synthase
Endothelin-1
Endothelins
Natriuretic Peptide, C-Type
Peptidyl-Dipeptidase A
RNA, Messenger
Receptors, Angiotensin
Renin