Korean J Otolaryngol-Head Neck Surg.  1998 Jul;41(7):876-882.

Expression of Epstein-Barr Virus in Inverted Papilloma by In Situ Hybridization and Polymerase Chain Reaction

• Kim JS
• Park NJ
• Park JS
• Lee SS
• Sohn EJ
• Shin SH

Abstract

BACKGROUND AND OBJECTIVES: The pathogenesis and etiology of inverted papilloma (IP) has not yet been clearly defined. The relationship between sinonasal IP and various strains of human papillomavirus (HPV) has been examined previously. Yet, there is little consensus regarding the incidence or role of HPV in IP. This study was performed to investigate the possible role of EBV in IP and to draw a relationship between histopathologic progression and EBV status.
MATERIALS AND METHODS
This study is comprised of 19 cases of IP and 10 cases of turbinate mucosa as control. To find out the presence of EBV in paraffin block, we used PCR and ISH. Also, the author examined coexisting areas of dysplasia or malignant transformation and compared histologic findings with the results of molecular biologic studies.
RESULTS
In PCR, the EBV genome was detected in 15 of 19 sinonasal IP (78.9%). By using ISH with the EBV oligonucleotide probe, EBV mRNA were found to be scattered throughout the epithelium in the IP with a similar incidence in PCR (13/19, 68.4.7%). One case of malignancy and all three dysplasia cases had EBV genome in PCR. In the middle turbinate mucosa which were used as control, 3/4 of the cases showed EBV genomes that are positive to PCR.
CONCLUSION
The above results imply that EBV plays a role in the pathogenesis of IP and also indicate that the middle turbinate might be a site of viral persistence.

Keyword

Inverted papilloma; Polymerase chain reaction; In situ hybridization; Epstein-Barr virus

MeSH Terms

Consensus
Epithelium
Genome
Herpesvirus 4, Human*
Humans
In Situ Hybridization*
Incidence
Mucous Membrane
Papilloma, Inverted*
Paraffin
Polymerase Chain Reaction*
RNA, Messenger
Turbinates
Paraffin
RNA, Messenger