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Delayed Influenza Treatment in Children With False-Negative Rapid Antigen Test: A Retrospective Single-Center Study in Korea 2016–2019

Lee JY, Baek SH, Ahn JG, Yoon SH, Kim MK, Kim SY, Kim KW, Sohn MH, Kang JM

Background: We aimed to examine the delay in antiviral initiation in rapid antigen test (RAT) false-negative children with influenza virus infection and to explore the clinical outcomes. We additionally conducted...
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Head-to-Head Comparison between Xpert MTB/RIF Assay and Real-Time Polymerase Chain Reaction Assay Using Bronchial Washing Specimens for Tuberculosis Diagnosis

Son E, Jang J, Kim T, Jang JH, Chung JH, Seol HY, Yeo HJ, Yoon SH, Lee SE, Cho WH, Kim YS, Jeon D

Background: With the introduction of Xpert MTB/RIF assay (Xpert), its incorporation into tuberculosis (TB) diagnostic algorithm has become an important issue. The aim of this study was to evaluate the...
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Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in the Pleural Fluid

Baek MS, Kim WY, Lee KJ, Noh CS

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be detected via a nasopharyngeal swab and in sputum, blood, urine, and feces. However, there is only limited data on the real-time...
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Developing species-specific quantitative real-time polymerase chain reaction primers for detecting Lautropia mirabilis

Park SN, Kook JK

This study aimed to develop Lautropia mirabilis-specific quantitative real-time polymerase chain reaction (qPCR) primers based on the sequence of DNA-directed RNA polymerase subunit beta gene. The PrimerSelect program was used...
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Diagnostic Evaluation of the BioFire ® Meningitis/Encephalitis Panel: A Pilot Study Including Febrile Infants Younger than 90 Days

Kim KM, Park JY, Park KU, Sohn YJ, Choi YY, Han MS, Choi EH

Purpose: Rapid detection of etiologic organisms is crucial for initiating appropriate therapy in patients with central nervous system (CNS) infection. This study aimed to evaluate the diagnostic value of the...
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A Patient with Immunocompetent Cryptococcal Meningitis Showing Repetitive False-Negative Immunoassay for Cryptococcal Antigen

Jung M, Oh H, Kim Kw, Kim MJ, Koo YS

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Molecular detection and characterization of ovine herpesvirus-2 using heminested PCR in Pakistan

Riaz A, Dry I, Dalziel R, Rehman SU, Shah MA, Akhtar HMN, Yousaf A, Baig R

Background: Malignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). Objectives:...
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Practical Diagnostic Approach to Myopathy

Lee JM

Hereditary myopathy is characterized by the weakness of skeletal muscles and is associated with various genetic defects. The efficiency of a genetic diagnosis has been archived with wide application of...
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Prevalence of Respiratory Viral Infection Using Multiplex Reverse Transcriptase-Polymerase Chain Reaction

Kim JS, Kim JM, Choi SK, Cho YJ, Kim SJ, Kim JA

Background: We wanted to understand the prevalence of respiratory viral infections in children hospitalized for acute respiratory infections stratified by sex, age, season, multiple infections, and clinical symptoms. Methods: Out of...
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Usefulness of Sputum and Pharyngeal Swab Specimens for Diagnosis of Coronavirus Disease 2019

Yun HS, Jang DS, Lee SH

Background: Various specimens are possible for the diagnosis of COVID-19, but previous studies comparing samples were small studies with hospitalized patients. The purpose of this study is to help diagnose...
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The Relationship of Leptin (+19) AG, Leptin (2548) GA, and Leptin Receptor Gln223Arg Gene Polymorphisms with Obesity and Metabolic Syndrome in Obese Children and Adolescents

Bilge S, Yılmaz R, Karaslan E, Özer S, Ateş , Ensari E, Demir O

Purpose: Obesity is defined as the abnormal or excessive accumulation of fat over acceptable limits. Leptin is a metabolic hormone present in the circulation in amounts proportional to fat mass....
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Pathologically and Genetically Diagnosed Subclinical Symptomatic Duchenne Muscular Dystrophy Carrier: Broadened Spectrum of Clinical Phenotype

Cho MS, Lee JM

A 29-year-old female presented with an elevated level of serum creatine kinase without subjective weakness. Neurologic examination showed the subtle motor weakness of the right arm. Muscle biopsy showed dystrophic...
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A comparative study of the prevalence of Helicobacter pylori in the oral biofilms of a group of dental and nondental undergraduates from Sri Lanka

Mallikaarachchi M, Rajapakse S, Gunawardhana K, Jayatilake J

Dental health care workers (DHCW) are at a risk of occupational exposure to Helicobacter pylori from the aerosolized oral biofilms and saliva of patients. We designed this study to investigate...
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Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl

Amiri A, Kashani MHG, Ghorbanian MT

Human adipose stem cells (hASCs) were introduced as appropriate candidate due to advantages like ease of isolation, in vitro expansion and lack of immune response. Deprenyl (Dep) was used to...
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Development of strain-specific polymerase chain reaction primers to detect Fusobacterium hwasookii strains

Lim YK, Kook JK

This study aimed to develop strain-specific polymerase chain reaction (PCR) primers to detect Fusobacterium hwasookii KCOM 1249T , F. hwasookii KCOM 1253, F. hwasookii KCOM 1256, F. hwasookii KCOM 1258,...
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Laboratory Diagnosis and Utilization for COVID-19

Kim MN, Kim HS, Bae HG, Huh HJ, Sung H

Since the outbreak of COVID-19 in March 2020, the COVID-19 diagnostic test has become a critical means of national response. Laboratory diagnosis of patients with COVID-19 is essential for not...
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Evaluation of Three Multiplex Realtime Reverse Transcription PCR Assays for Simultaneous Detection of SARS-CoV-2, Influenza A/B, and Respiratory Syncytial Virus in Nasopharyngeal Swabs

Yun J, Park JH, Kim N, Roh EY, Shin S, Yoon JH, Kim TS, Hyunwoong H

Background: In the coronavirus disease 2019 (COVID-19) pandemic era, the simultaneous detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza virus (Flu), and respiratory syncytial virus (RSV) is important...
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Recognition of the Y chromosome in Turner syndrome using peripheral blood or oral mucosa tissue

Barbosa LG, Siviero-Miachon AA, Souza MA, Spinola-Castro AM

Purpose: Turner syndrome is defined as total or partial loss of the second sex chromosome in a phenotypically female patient. Due to the possibility of hidden mosaicism of fragments of...
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Serial Screening for SARS-CoV-2 in Rectal Swabs of Symptomatic COVID-19 Patients

Jung SH, Kim SW, Lee H, Oh JH, Lim J

We used serial rectal swabs to investigate the amount and duration of virus secretion through the gastrointestinal tract and assessed the association between fecal shedding and gastrointestinal symptoms and to...
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Effectiveness of Cytochrome c Oxidase Subunit 1 Gene Nested Polymerase Chain Reaction Compared to Dermoscopy or Microscopy Alone for the Detection and Diagnosis of Sarcoptes scabiei var. hominis Infection

Yang SY, Kim SS, Kim CW

Background: While microscopy (MS) evaluation of skin scrapings has a 100% positive predictive value and specificity by definition for scabies diagnosis, it has low sensitivity. Dermoscopy (DS) has not yet...
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