J Korean Ophthalmol Soc.  2008 Jul;49(7):1135-1145. 10.3341/jkos.2007.49.7.1135.

The Role of Endothelial Progenital Cells and Fibrin on Vascularization and Stability in Orbital Implant

• Yang JW
• Lee HY
• Park SG
• Yang YI

• KMID: 1476969
• DOI: http://doi.org/10.3341/jkos.2007.49.7.1135

Abstract

PURPOSE: The effects of endothelial progenitor cells (EPCs) and fibrin on fibrovascular growth into porous polyethylene orbital implants (Medpor(R) sheet) were investigated using stem cells.
METHODS
EPCs were separated from human adipose fat tissue for culture. Fluorescence-activated cell sorting (FACS) was used to identify the phenotype and to analyze the purity of EPCs cultivated from human adipose tissue. Processed Medpor(R) sheets were inserted in each quadrant of the subcutaneous fat layer under the dorsal surface of 20 anesthetized athymic nude mice, using sterile methods. Medpor(R) sheets processed with endothelial progenitor cells and fibrin were inserted into the two top quadrants, a Medpor(R) sheet processed with fibrin was inserted in the lower right quadrant, and an unprocessed Medpor(R) sheet was inserted in the lower left quadrant of each mouse. The mice were sacrificed on the seventh day. The adhesiveness and blood vessel formation were quantified by weight and the number of blood cells within the Medpor(R) sheets. Hematoxylin and eosin (H&E) and toluidine blue stains were used to analyze fibrovascular and cell growth within the Medpor(R) sheets.
RESULTS
The sheets processed with EPCs and fibrin were heavier and contained more white and red blood cells (p<0.001) than the other sheets. The sheets processed with fibrin alone were heavier (p<0.01) and contained more blood cells (p<0.001) than the unprocessed sheets. The degree of vessel formation and tissue adhesiveness was greatest in the group of Medpor(R) sheets processed with EPCs and fibrin. The sheets processed with fibrin only had greater tissue adhesiveness and fibrovascular proliferation than the unprocessed Medpor(R) sheets.
CONCLUSIONS
Endothelial progenitor cells and fibrin applied to Medpor(R) sheets improve fibrovascular proliferation and tissue adhesiveness. When both are applied together, a synergistic effect is seen.

Keyword

Endothelial progenital cells; Fibrin; Fibrovascularization; Medpor(R) sheet

MeSH Terms

Adhesiveness
Adipose Tissue
Animals
Blood Cells
Blood Vessels
Coloring Agents
Eosine Yellowish-(YS)
Erythrocytes
European Continental Ancestry Group
Fibrin
Flow Cytometry
Glycosaminoglycans
Hematoxylin
Humans
Mice
Mice, Nude
Orbit
Orbital Implants
Phenotype
Polyethylene
Stem Cells
Subcutaneous Fat
Tolonium Chloride
Coloring Agents
Eosine Yellowish-(YS)
Fibrin
Glycosaminoglycans
Hematoxylin
Polyethylene
Tolonium Chloride