J Korean Soc Coloproctol.  2012 Jun;28(3):132-139. 10.3393/jksc.2012.28.3.132.

Adipose-tissue-derived Stem Cells Enhance the Healing of Ischemic Colonic Anastomoses: An Experimental Study in Rats

• Yoo JH
• Shin JH
• An MS
• Ha TK
• Kim KH
• Bae KB
• Kim TH
• Choi CS
• Hong KH
• Kim J
• Jung SJ
• Kim SH
• Rho KH
• Kim JT
• Yang YI

• KMID: 1438190
• DOI: http://doi.org/10.3393/jksc.2012.28.3.132

Abstract

PURPOSE
This experimental study verified the effect of adipose-tissue-derived stem cells (ASCs) on the healing of ischemic colonic anastomoses in rats.
METHODS
ASCs were isolated from the subcutaneous fat tissue of rats and identified as mesenchymal stem cells by identification of different potentials. An animal model of colonic ischemic anastomosis was induced by modifying Nagahata's method. Sixty male Sprague-Dawley rats (10-week-old, 370 +/- 50 g) were divided into two groups (n = 30 each): a control group in which the anastomosis was sutured in a single layer with 6-0 polypropylene without any treatment and an ASCtreated group (ASC group) in which the anastomosis was sutured as in the control group, but then ASCs were locally transplanted into the bowel wall around the anastomosis. The rats were sacrificed on postoperative day 7. Healing of the anastomoses was assessed by measuring loss of body weight, wound infection, anastomotic leakage, mortality, adhesion formation, ileus, anastomotic stricture, anastomotic bursting pressure, histopathological features, and microvascular density.
RESULTS
No differences in wound infection, anastomotic leakage, or mortality between the two groups were observed. The ASC group had significantly more favorable anastomotic healing, including less body weight lost, less ileus, and fewer ulcers and strictures, than the control group. ASCs augmented bursting pressure and collagen deposition. The histopathological features were significantly more favorable in the ASC group, and microvascular density was significantly higher than it was in the control group.
CONCLUSION
Locally-transplanted ASCs enhanced healing of ischemic colonic anastomoses by increasing angiogenesis. ASCs could be a novel strategy for accelerating healing of colonic ischemic risk anastomoses.

Keyword

Colonic anastomosis; Ischemia; Anastomotic healing; Adipose-tissue-derived stem cell; Angiogenesis

MeSH Terms

Anastomotic Leak
Animals
Body Weight
Collagen
Colon
Constriction, Pathologic
Humans
Ileus
Ischemia
Male
Mesenchymal Stromal Cells
Models, Animal
Polypropylenes
Rats
Rats, Sprague-Dawley
Stem Cells
Subcutaneous Fat
Transplants
Ulcer
Wound Infection
Collagen
Polypropylenes