J Korean Assoc Oral Maxillofac Surg.  2010 Oct;36(5):392-401.

Effect of adipose-derived stem cells on bone healing on titanium implant in tibia of diabetes mellitus induced rats

• Kim MG
• Jung IK
• Shin SH
• Kim CH
• Kim BJ
• Kim JH
• Hwang YS
• Jung EG
• Kim JW
• Kim UK

Abstract

INTRODUCTION
Diabetes mellitus, as a major health problem for the elderly has been shown to alter the properties of the bone and impair bone healing around a titanium implant in both humans and animals. The aim of this study was to examine the effect of adipose-derived stem cells on the healing process around a titanium implant in streptozotocin-induced diabetic rats.
MATERIALS AND METHODS
Thirteen rats were divided into two groups: adipose-derived stem cells injected group and a control group. A titanium screw implant (diameter: 2.0 mm, length: 3.5 mm) was placed into both tibia of 13 rats: 13 right tibia as the control group and 13 left tibia as the experimental group. The rats were sacrificed at different intervals (1, 2, and 4 weeks) after implantation for histopathology observations and immunohistochemistric analysis.
RESULTS
The histopathological findings revealed earlier new formed bone in the experimental group than the control group. In particular, at 1 week after implantation, the experimental group showed more newly formed bone and collagen around the implant than the control group. In immunohistochemistric analysis, osteoprotegerin (OPG) expression in the experimental group increased early compared to that of the control group until 2 weeks after implantation. However, after 2 weeks, OPG expression in the experimental group was similar to OPG expression in the control group. The receptor activator of nuclear factor kappaB ligand (RANKL) expression in the experimental group increased early compared to that of the control group, and then decreased at 2 weeks. After 2 weeks, the level of RANKL expression was similar in both groups.
CONCLUSION
These results suggest that adipose-derived stem cells in implantation can promote bone healing around titanium, particularly in diabetes mellitus induced animals.

Keyword

Stem cell; Dental implants; Diabetes mellitus; Rats

MeSH Terms

Aged
Animals
Collagen
Dental Implants
Diabetes Mellitus
Humans
Osteoprotegerin
RANK Ligand
Rats
Stem Cells
Tibia
Titanium
Collagen
Dental Implants
Osteoprotegerin
RANK Ligand
Titanium

Figure

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  Fig. 1. Blood sugar level test.

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  Fig. 2. Microphotograph of stem cell (Original magnification×100).

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  Fig. 3. Photo of stem cell injection.

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  Fig. 4. Photo of Implantation.

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  Fig. 5. Microphotograph at 1 week after implantation in control rat.(H&E staining, original magnification ×100)

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  Fig. 6. Microphotograph at 2 weeks after implantation in control rat.(H&E staining, original magnification ×100)

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  Fig. 7. Microphotograph at 4 weeks after implantation in control rat.(H&E staining, original magnification ×100)

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  Fig. 8. Microphotograph at 1 week after implantation in experimental rat.(H&E staining, original magnification ×100)

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  Fig. 9. Microphotograph at 2 weeks after implantation in experimental rat.(H&E staining, original magnification ×100)

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  Fig. 10. Microphotograph at 4 weeks after implantation in experimental rat.(H&E staining, original magnification ×100)

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  Fig. 11. Microphotograph at 1 week after implantation in control rat. (Masson's trichrome stain. Original magnification ×100)

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  Fig. 12. Microphotograph at 2 weeks after implantation in control rat. (Masson's trichrome stain. Original magnification ×100)

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  Fig. 13. Microphotograph at 4 weeks after implantation in control rat. (Masson's trichrome stain. Original magnification ×100)

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  Fig. 14. Microphotograph at 1 week after implantation in experimental rat. (Masson's trichrome stain. Original magnification ×100)

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  Fig. 15. Microphotograph at 2 weeks after implantation in experimental rat. (Masson's trichrome stain. Original magnification ×100)

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  Fig. 16. Microphotograph at 4 weeks after implantation in experimental rat. (Masson's trichrome stain. Original magnification ×100)

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  Fig. 17. Osteoproteogrin antibody reaction of regenerating bone at 1 week after implantation on tibia of the control rat. ×200.

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  Fig. 18. Osteoproteogrin antibody reaction of regenerating bone at 2 weeks after implantation on tibia of the control rat. ×200.

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  Fig. 19. Osteoproteogrin antibody reaction of regenerating bone at 1 week after implantation on tibia of the Experimental rat. ×200.

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  Fig. 20. Osteoproteogrin antibody reaction of regenerating bone at 2 weeks after implantation on tibia of the Experimental rat. ×200.

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  Fig. 21. Receptor activator of nuclear factor kB ligand antibody reaction of regenerating bone at 1 weeks after implantation on tibia of the Control rat. ×200.

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  Fig. 22. Receptor activator of nuclear factor kB ligand antibody reaction of regenerating bone at 2 weeks after implantation on tibia of the Control rat. ×200.

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  Fig. 23. Receptor activator of nuclear factor kB ligand antibody reaction of regenerating bone at 1 weeks after implantation on tibia of the Experimental rat. ×200.

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  Fig. 24. Receptor activator of nuclear factor kB ligand antibody reaction of regenerating bone at 2 weeks after implantation on tibia of the Experimental rat. ×200.

Reference

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