Lab Anim Res.  2012 Sep;28(3):165-170. 10.5625/lar.2012.28.3.165.

Comparison of alpha-synuclein immunoreactivity in the spinal cord between the adult and aged beagle dog

Affiliations
  • 1Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation Science, Daegu University, Gyeongsan, Korea.
  • 2Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea. mhwon@kangwon.ac.kr
  • 3Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Korea.
  • 4Department of Physiology, College of Medicine, and Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon, Korea. hcshin@hallym.ac.kr

Abstract

Alpha-synuclein (alpha-syn) is a presynaptic protein that is richly expressed in the central and peripheral nervous systems of mammals, and it is related to the pathogenesis of Parkinson's disease and other neurodegenerative disorders. In the present study, we compared the distribution of the immunoreactivity of alpha-syn and its related gliosis in the spinal cord of young adult (2-3 years) and aged (10-12 years) beagle dogs. We discovered that alpha-syn immunoreactivity was present in many neurons in the thoracic level of the aged spinal cord, however, its protein level was not distinct inform that of the adult spinal cord. In addition, ionized calcium-binding adapter molecule-1 (a marker for microglia) immunoreactivity, and not glial fibrillary acidic protein (a marker for astrocytes) immunoreactivity, was somewhat increased in the aged group compared to the adult group. These results indicate that alpha-syn immunoreactivity was not dramatically changed in the dog spinal cord during aging.

Keyword

Aging; neuroprotein; spinal gray matter; nerve terminals and fibers; alpha-synuclein (alpha-syn)
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