Korean J Ophthalmol.  2006 Sep;20(3):162-165. 10.3341/kjo.2006.20.3.162.

Transforming Growth Factor-beta Levels in Human Aqueous Humor of Glaucomatous, Diabetic and Uveitic Eyes

• Min SH
• Lee TI
• Chung YS
• Kim HK

• KMID: 754573
• DOI: http://doi.org/10.3341/kjo.2006.20.3.162

Abstract

PURPOSE: Transforming growth factor-beta2 is known to be present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG) and diabetes but not in uveitis-related secondary glaucoma. We investigated total TGF-beta2 levels and levels of the active form of TGF-beta2 in the aqueous humor of eyes with different types of glaucoma. METHODS: The concentration of the total and active form of TGF-beta2 was measured in 63 patients with primary open angle glaucoma, neovascular glaucoma complicated with diabetes (NVG), and secondary open angle glaucoma complicated with uveitis (SOAG) using a double antibody 'sandwich-indirect' ELISA method. RESULTS: The levels of total TGF-beta2 in the aqueous samples of POAG, NVG, and SOAG were elevated. The levels of active TGF-beta2 in the aqueous samples of POAG, and NVG were also elevated, whereas the level of active TGF-beta2 was within the normal range in the aqueous samples of SOAG. CONCLUSIONS: These results suggest that the level of TGF-beta2 may play a role in the pathology of various types of glaucoma.

Keyword

Aqueous humor; Neovascular glaucoma; Primary open angle glaucoma; Secondary open angle glaucoma; Transforming growth factor-beta

MeSH Terms

Uveitis/*metabolism
Transforming Growth Factor beta/*metabolism
Severity of Illness Index
Middle Aged
Humans
Glaucoma, Open-Angle/*metabolism
Glaucoma, Angle-Closure/*metabolism
Enzyme-Linked Immunosorbent Assay
Diabetic Retinopathy/*metabolism
Biological Markers/metabolism
Aqueous Humor/*metabolism
Aged, 80 and over
Aged
Adult

Figure

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  Fig. 1 Concentration of total transforming growth factor (TGF)-β2 in the aqueous humor of different types of glaucoma.

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  Fig. 2 Concentration of active transforming growth factor (TGF)-β2 in the aqueous humor of different types of glaucoma.

Reference

1. Imanishi J, Kamiyama K, Iguchi I, et al. Growth factors: importance in wound healing and maintenance of transparency of the cornea. Prog Retin Eye Res. 2000. 19:113–129.

2. Kokawa N, Sotozono C, Nishida K, Kinoshita S. High total TGF-b levels in normal human tears. Curr Eye Res. 1996. 15:341–343.

3. Roberts AB, Sporn MB. Sporn MB, Roberts AB, editors. The transforming growth factor-βs. Handbook of Experimental Phamacology. 1990. 95. Heidelberg: Springer-Veriag;419–472.

4. Nishida K, Sotozono C, Adachi W, et al. Transforming growth factor-beta 1, -beta 2, and -beta 3 mRNA expression in human cornea. Curr Eye Res. 1995. 14:235–241.

5. Jampel HD, Roche N, Stark WJ, Roberts AB. Transforming growth factor-β in human aqueous humor. Eye Res. 1990. 9:963–969.

6. Tanihara H, Inatani M, Honda Y. Growth factor and their receptors in the retina and pigment epithelium. Prog Ret Eye Res. 1997. 16:271–301.

7. Inatani M, Tanihara H, Katsuta H, et al. Transforming growth factor-β₂ in aqueous humor of glaucomatous eyes. Graefe's Arch Clin Exp Ophthalmol. 2001. 239:109–113.

8. Boer JH, Limpens J, Orengo-Namia S, et al. Low mature TGF-β₂ in aqueous humor during uveitis. Invest Ophthalmol Vis Sci. 1994. 35:3702–3710.

9. Ochiai Y, Ochiai H. Higher concentration of transforming growth factor-β in aqueous humor of glaucomatous eyes and diabetic eyes. Jpn J Ophthalmol. 2002. 46:249–253.

10. Tripathi RC, Li J, Chan WF, Tripathi BJ. Aqueous humor in glaumatous eyes conatains an increased level of TGF-β₂. Exp Eye Res. 1994. 9:723–728.

11. Cousins SW, McCabe MM, Danielpour D, Streilein JW. Identification of transforming growth factor-betas as an immunosuppressive factor in aqueous humor. Invest Ophthalmol Vis Sc. 1991. 32:2201–2211.

12. Yamashita H. Functions of the transforming grouth factor-β superfamily in eyes. J Jpn Ophthalmol Soc. 1997. 101:927–947.

13. Borisuth NSC, Tripathi BJ, Tripathi RC. Identification and partial characterization of TGF-β₁ receptors on trabeculat cells. Invest Ophthalmol Vis Sci. 1992. 3:407–414.

14. Hirase K, Sotozono C, Ikeda T, et al. Transforming growth factor beta2 in the vitreous in proliferative diabetic retinopathy. Arch Ophthalmol Vis Sci. 1998. 116:738–741.