Lab Anim Res.  2019 Aug;35(2):52-60. 10.1186/s42826-019-0009-x.

Metabolomics approach to serum biomarker for laxative effects of red Liriope platyphylla in loperamide-induced constipation of SD rats

  • 1Department of Biomaterials Science, College of Natural Resources & Life Science / Industry Convergence Research Institute, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup Miryang-si, Gyeongsangnam-do 627-706, South Korea.
  • 2Analysis Research Team, R&D Center, GL Pharm Tech Corp, Gyeonggi-do 13202, Republic of Korea.
  • 3Laboratory of Veterinary Theriogenology, Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South Korea.
  • 4College of Pharmacy, Dankook University, Chungnam 330-714, South Korea.
  • 5Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 609-735, South Korea.
  • 6Department of Clinical Laboratory Science, College of Nursing and Healthcare Science, Dong-Eui University, Busan, South Korea.


Red Liriope platyphylla (RLP) is a known herbal medicine used in the treatment of some chronic diseases including constipation, neurodegenerative disorders, diabetes and obesity. To determine and characterize putative biomarkers that predict the laxative effects induced by RLP treatment, alteration of endogenous metabolites was measured in the serum of loperamide (Lop)-induced constipation rats after administration of RLP extract (EtRLP) using 1H nuclear magnetic resonance (1H NMR) spectral data. The urine volume and amounts, and weights and water contents of stools were significantly recovered in the Lop + EtRLP treated group as compared to the No group, whereas body weight and food intake maintained constant levels. Also, significant recoveries in the thickness of mucosa and muscle were detected in the colon of the Lop + EtRLP treated group. Furthermore, pattern recognition showed absolutely different clustering of the serum analysis parameters when comparing the Lop treated group and Lop + EtRLP treated group. Of the 33 endogenous metabolites, 7 amino acids (alanine, arginine, glutamate, glutamine, glycine, threonine and valine) and 8 endogenous metabolites (betaine, creatine, glucose, taurine, ethanol, lactate, glycerol and succinate) were dramatically increased in the Lop + EtRLP treated SD rats. These results provide the first evidence pertaining to metabolic changes in the constipation rats treated with Lop + EtRLP. Additionally, these findings correlate with changes observed in 15 metabolites during the laxative effects of EtRLP.


Red Liriope platyphylla; Constipation; Laxative effects; Metabolomics; Serum
Full Text Links
  • LAR
export Copy
  • Twitter
  • Facebook
Similar articles
Copyright © 2021 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: