Endocrinol Metab.  2019 Sep;34(3):302-313. 10.3803/EnM.2019.34.3.302.

Effects of Oxytocin on Cell Proliferation in a Corticotroph Adenoma Cell Line

Affiliations
  • 1Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
  • 2Institute of Evidence-based Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
  • 3Cell Therapy and Tissue Engineering Center, Yonsei University Wonju College of Medicine, Wonju, Korea.
  • 4Department of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea.
  • 5Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea.
  • 6Yonsei Institute of Pharmaceutical Sciences, Yonsei University College of Pharmacy, Incheon, Korea.
  • 7Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. ejlee423@yuhs.ac

Abstract

BACKGROUND
Oxytocin (OXT) has been reported to act as a growth regulator in various tumor cells. However, there is a paucity of data on the influence of OXT on cell proliferation of corticotroph adenomas. This study aimed to examine whether OXT affects cell growth in pituitary tumor cell lines (AtT20 and GH3 cells) with a focus on corticotroph adenoma cells.
METHODS
Reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were conducted with AtT20 cells to confirm the effects of OXT on hormonal activity; flow cytometry was used to assess changes in the cell cycle after OXT treatment. Moreover, the impact of OXT on proliferating cell nuclear antigen (PCNA), nuclear factor κB, and mitogen-activated protein kinase signaling pathway was analyzed by Western blot.
RESULTS
OXT treatment of 50 nM changed the gene expression of OXT receptor and pro-opiomelanocortin within a short time. In addition, OXT significantly reduced adrenocorticotropic hormone secretion within 1 hour. S and G2/M populations of AtT20 cells treated with OXT for 24 hours were significantly decreased compared to the control. Furthermore, OXT treatment decreased the protein levels of PCNA and phosphorylated extracellular-signal-regulated kinase (P-ERK) in AtT20 cells.
CONCLUSION
Although the cytotoxic effect of OXT in AtT20 cells was not definite, OXT may blunt cell proliferation of corticotroph adenomas by altering the cell cycle or reducing PCNA and P-ERK levels. Further research is required to investigate the role of OXT as a potential therapeutic target in corticotroph adenomas.

Keyword

ACTH-secreting pituitary adenoma; Oxytocin; Corticotrophs; Cell proliferation

MeSH Terms

ACTH-Secreting Pituitary Adenoma*
Adrenocorticotropic Hormone
Blotting, Western
Cell Cycle
Cell Line*
Cell Proliferation*
Corticotrophs*
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Gene Expression
Oxytocin*
Phosphotransferases
Pituitary Neoplasms
Polymerase Chain Reaction
Pro-Opiomelanocortin
Proliferating Cell Nuclear Antigen
Protein Kinases
Reverse Transcription
Adrenocorticotropic Hormone
Oxytocin
Phosphotransferases
Pro-Opiomelanocortin
Proliferating Cell Nuclear Antigen
Protein Kinases
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