J Korean Gastric Cancer Assoc.  2006 Sep;6(3):132-138.

Roles of Dopamine in Proliferation of Gastric-Cancer Cells

Affiliations
  • 1Department of Surgery, Catholic University of Daegu School of Medicine, Daegu, Korea. saxochea@hanmail.net

Abstract

PURPOSE: Dopamine is a neurotransmitter, but in the GIT, the roles of dopamine are a regulator of epithelial cell proliferation, an endogenous protective factor, and a regulator of stomach cancer cell proliferation. By using two different gastric-cancer cell lines, we assessed the effects of dopamine and dopamine receptors on the proliferation of human gastric-cancer cells.
MATERIALS AND METHODS
To assess the effects of dopamine and dopamine receptors on the proliferation of human gastric-cancer cells, we investigated cell proliferation and the expression of D1, D2L, and D2S receptor in two gastric-cancer cell lines, SNU 601 and KCU-C2. The effects of dopamine and dopamine receptors on the level of the cell proliferation were determined by staining with an A/H/E (acridine orange, hoechst and ethidium bromide) mixture.
RESULTS
After dopamine treatment, the cell viability was significantly decreased in SNU 601 cells (P<0.05) where the D2L receptor was absent, but not in KCU-C2 cells. After treatment with raclopride, a D2 receptor antagonist, dopamine-dose-dependent inhibition of cell proliferation was observed in SNU 601 cells (P<0.05). After treatment with SCH 23390, a D1 receptor antagonist, dopamine significantly increased cell proliferation in KCU-C2 cells (P<0.05), but inhibited cell proliferation in SNU 601 cells (no D2L receptor).
CONCLUSION
The dopamine signal via the D1 or the D2S receptor inhibited proliferation of gastric-cancer cells, but that via the D2L receptor increased proliferation. These results suggest that the regulatory effects of dopamine in the gastric-cancer cell proliferation may be controlled by using dopamine receptors.

Keyword

Dopamine; Gastric cancer; Gastric-cancer cell line; Dopamine receptors

MeSH Terms

Cell Line
Cell Proliferation
Cell Survival
Citrus sinensis
Dopamine*
Epithelial Cells
Ethidium
Humans
Neurotransmitter Agents
Raclopride
Receptors, Dopamine
Stomach Neoplasms
Dopamine
Ethidium
Neurotransmitter Agents
Raclopride
Receptors, Dopamine
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