Korean J Pain.  2019 Apr;32(2):87-96. 10.3344/kjp.2019.32.2.87.

Effect of sec-O-glucosylhamaudol on mechanical allodynia in a rat model of postoperative pain

Affiliations
  • 1Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
  • 2Department of Anesthesiology and Pain Medicine, Chosun University Hospital, Gwangju, Korea. mdmole@chosun.ac.kr
  • 3Department of Anesthesiology and Pain Medicine, School of Medicine, Chosun University, Gwangju, Korea.
  • 4Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University, Gwangju, Korea.
  • 5School of Medicine, Chosun University, Gwangju, Korea.

Abstract

BACKGROUND
This study was performed in order to examine the effect of intrathecal sec-O-glucosylhamaudol (SOG), an extract from the root of the Peucedanum japonicum Thunb., on incisional pain in a rat model.
METHODS
The intrathecal catheter was inserted in male Sprague-Dawley rats (n = 55). The postoperative pain model was made and paw withdrawal thresholds (PWTs) were evaluated. Rats were randomly treated with a vehicle (70% dimethyl sulfoxide) and SOG (10 μg, 30 μg, 100 μg, and 300 μg) intrathecally, and PWT was observed for four hours. Dose-responsiveness and ED50 values were calculated. Naloxone was administered 10 min prior to treatment of SOG 300 μg in order to assess the involvement of SOG with an opioid receptor. The protein levels of the δ-opioid receptor, κ-opioid receptor, and μ-opioid receptor (MOR) were analyzed by Western blotting of the spinal cord.
RESULTS
Intrathecal SOG significantly increased PWT in a dose-dependent manner. Maximum effects were achieved at a dose of 300 μg at 60 min after SOG administration, and the maximal possible effect was 85.35% at that time. The medial effective dose of intrathecal SOG was 191.3 μg (95% confidence interval, 102.3-357.8). The antinociceptive effects of SOG (300 μg) were significantly reverted until 60 min by naloxone. The protein levels of MOR were decreased by administration of SOG.
CONCLUSIONS
Intrathecal SOG showed a significant antinociceptive effect on the postoperative pain model and reverted by naloxone. The expression of MOR were changed by SOG. The effects of SOG seem to involve the MOR.

Keyword

Analgesia; Blotting, western; Dimethyl sulfoxide; Hyperalgesia; Nociceptive pain; Pain, postoperative; Rats; Receptors, opioid; Spinal cord; 11-hydroxy-sec-O-glocosylhamaudol

MeSH Terms

Analgesia
Animals
Blotting, Western
Catheters
Dimethyl Sulfoxide
Humans
Hyperalgesia*
Male
Models, Animal*
Naloxone
Nociceptive Pain
Pain, Postoperative*
Rats*
Rats, Sprague-Dawley
Receptors, Opioid
Spinal Cord
Dimethyl Sulfoxide
Naloxone
Receptors, Opioid
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