J Vet Sci.  2019 May;20(3):e31. 10.4142/jvs.2019.20.e31.

Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment

• Kim G
• Roy PK
• Fang X
• Hassan BM
• Cho J

• KMID: 2448715
• DOI: http://doi.org/10.4142/jvs.2019.20.e31

Abstract

This study examined the effects of a caffeine treatment to improve nuclear reprogramming in porcine cloned embryos. Embryonic development and the expression of genes related to pluripotency (POU5F1, SOX2, NANOG, and CDX2) were compared after caffeine supplementation during manipulation at different concentrations (0, 1.25, 2.5, and 5.0 mM) and after varying the delayed activation time (control, 1, 2, and 4 h) after fusion. Caffeine added to media during manipulation produced a higher rate of development to blastocysts in the 1.25 mM group than in the other concentration groups (22.8% vs. 16.1%, 16.2%, and 19.2%; p < 0.05). When caffeine was added during the 4 h delayed activation, the 1.25 mM caffeine concentration produced a significantly higher rate of development than those in the other 4 h-activation-delayed caffeine concentration groups (22.4% vs. 9.4%, 14.0%, and 11.1%; p < 0.05). On the other hand, no significant improvement over that in the control group was observed when caffeine was supplemented during both the manipulation period and delayed activation period (16.0% vs. 15.2%), respectively. The levels of POU5F1, SOX2, and NANOG expression in blastocysts were significantly higher in the delayed activation caffeine group (4 h, 1.25 mM) than in the control group (1 h, 0 mM; p < 0.05). In conclusion, a caffeine treatment at 1.25 mM during delayed activation for 4 h can improve the preimplantation development of porcine somatic cell nuclear transfer embryos by activating nuclear reprogramming.

Keyword

Caffeine; development; embryo; porcine; somatic cell nuclear transfer

MeSH Terms

Blastocyst
Caffeine*
Cellular Reprogramming
Clone Cells
Embryonic Development
Embryonic Structures*
Female
Hand
Pregnancy
Caffeine