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Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles

Lee H, Lee Y, Lee J, Lee GS, Lee ST, Lee E

This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth, maturation, and parthenogenesis (PA) of pig oocytes derived from small...
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Improved preimplantation development of porcine somatic cell nuclear transfer embryos by caffeine treatment

Kim G, Roy PK, Fang X, Hassan BM, Cho J

This study examined the effects of a caffeine treatment to improve nuclear reprogramming in porcine cloned embryos. Embryonic development and the expression of genes related to pluripotency (POU5F1, SOX2, NANOG,...
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Effects of paternal age on human embryo development in in vitro fertilization with preimplantation genetic screening

Kim MK, Park JK, Jeon Y, Seok SH, Chang EM, Lee WS

OBJECTIVE: As paternal age increases, the quality of sperm decreases due to increased DNA fragmentation and aneuploidy. Higher levels of structural chromosomal aberrations in the gametes ultimately decrease both the...
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Effects of dynamic oxygen concentrations on the development of mouse pre- and peri-implantation embryos using a double-channel gas supply incubator system

Lee SC, Seo HC, Lee J, Jun JH, Choi KW

OBJECTIVE: We aimed to evaluate the effects of different oxygen conditions (20% [high Oâ‚‚], 5% [low Oâ‚‚] and 5% decreased to 2% [dynamic Oâ‚‚]) on mouse pre- and peri-implantation development...
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Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts

Chatroudi , Khalili MA, Ashourzadeh S, Anbari F, Shahedi A, Safari S

OBJECTIVE: In vitro maturation (IVM) of immature oocytes can be useful for some infertile patients. In IVM programs, the rates of embryo formation and pregnancy are low. Therefore, it is...
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Correlation between Morphologic Grading and Euploidy Rates of Blastocysts, and Clinical Outcomes in In Vitro Fertilization Preimplantation Genetic Screening

Kim MK, Park JK, Jeon Y, Choe SA, Lee HJ, Kim J, Chang EM, Kim JW, Lyu SW, Kim JY, Kwak IP, Lee WS, Yoon TK

BACKGROUND: The standard morphological evaluation has been widely used for embryo selection, but it has limitations. This study aimed to investigate the correlation between morphologic grading and euploidy rate of...
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Rapid freezing versus Cryotop vitrification of mouse two-cell embryos

Inna N, Sanmee U, Saeng-anan U, Piromlertamorn W, Vutyavanich T

OBJECTIVE: To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. METHODS: Two-cell mouse embryos...
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Retrospective study of single vitrified-warmed blastocyst transfer cycles according to the presence of morphokinetic variables

Hur YS, Ryu EK, Hyun CS, Yang SH, Yoon SH, Lim KS, Lee WD, Lim JH

This study retrospectively assessed whether time-lapse data relating to developmental timing and morphology were associated with clinical outcomes, with the eventual goal of using morphokinetic variables to select embryos prospectively...
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Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

Pongsuthirak P, Vutyavanich T

OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes...
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Pre-implantation genetic diagnosis and pre-implantation genetic screening: two years experience at a single center

Won SY, Kim H, Lee WS, Kim JW, Shim SH

OBJECTIVE: Indications for preimplantation genetic diagnosis (PGD)/preimplantation genetic screening (PGS) cycles and clinical outcomes were evaluated at CHA Gangnam Medical Center. METHODS: This is retrospective cohort study. All patients (n=336) who...
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The association of follicular fluid volume with human oolemma stretchability during intracytoplasmic sperm injection

Inoue T, Yamashita , Tsujimoto , Yamamoto S, Taguchi S, Hirao K, Uemura M, Ikawa K, Miyazaki K

OBJECTIVE: Oocyte degeneration often occurs after intracytoplasmic sperm injection (ICSI), and the risk factor is low-quality oocytes. The follicular fluid (FF) provides a crucial microenvironment for oocyte development. We investigated...
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Addition of interleukin-6 to mouse embryo culture increases blastocyst cell number and influences the inner cell mass to trophectoderm ratio

Kelley RL, Gardner DK

OBJECTIVE: In vitro culture of preimplantation embryos is improved by grouping embryos together in a drop of media. Individually cultured embryos are deprived of paracrine factors; with this in mind,...
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Mixed double-embryo transfer: A promising approach for patients with repeated implantation failure

Stamenov G, Parvanov D, Chaushev T

OBJECTIVE: The purpose of this study was to evaluate the efficacy of frozen mixed double-embryo transfer (MDET; the simultaneous transfer of day 3 and day 5 embryos) in comparison with...
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Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study

Kim HJ, Yoon HJ, Jang JM, Lee WD, Yoon SH, Lim JH

OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830...
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Influence of the insemination method on the outcomes of elective blastocyst culture

Wang C, Feng G, Zhang B, Shu J, Zhou H, Gan X, Lin R

OBJECTIVE: The aim of this study was to explore the effects of the insemination method on the outcomes of elective blastocyst culture. METHODS: We retrospectively analyzed the outcomes of elective blastocyst...
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The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles

Fesahat F, Dehghani Firouzabadi R, Faramarzi , Khalili MA

OBJECTIVE: Optimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate...
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Oocyte maturation under a biophoton generator improves preimplantation development of pig embryos derived by parthenogenesis and somatic cell nuclear transfer

Lee J, Shin H, Lee W, Lee ST, Lee GS, Hyun SH, Lee E

This study was conducted to determine the effects of biophoton treatment during in vitro maturation (IVM) and/or in vitro culture (IVC) on oocyte maturation and embryonic development in pigs. An...
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Reactive oxygen species-mediated unfolded protein response pathways in preimplantation embryos

Ali I, Shah SZ, Jin Y, Li ZS, Ullah O, Fang NZ

Excessive production of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress-mediated responses are critical to embryonic development in the challenging in vitro environment. ROS production increases during early embryonic...
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Pathogenic variant in NLRP7 (19q13.42) associated with recurrent gestational trophoblastic disease: Data from early embryo development observed during in vitro fertilization

Sills ES, Obregon-Tito AJ, Gao H, McWilliams TK, Gordon AT, Adams CA, Slim R

OBJECTIVE: To describe in vitro development of human embryos derived from an individual with a homozygous pathogenic variant in NLRP7 (19q13.42) and recurrent hydatidiform mole (HM), an autosomal recessive condition...
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Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Yoon J, Juhn KM, Yoon SH, Ko Y, Lim JH

OBJECTIVE: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM),...
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