J Korean Acad Pediatr Dent.  2019 Feb;46(1):85-92. 10.5933/JKAPD.2019.46.1.85.

Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth

Affiliations
  • 1Department of Pediatric Dentistry, College of Dentistry, Wonkwang University, Korea. 9543sue@hanmail.net
  • 2Department of Oral Biochemistry, College of Dentistry, Wonkwang University, Korea.
  • 3Department of Dental Hygiene, Kyungbok University, Korea.
  • 4Department of Oral and Maxillofacial Surgery, College of Dentistry, Wonkwang University, Korea.
  • 5Wonkwang Bone Regeneration Research Institute, Korea.

Abstract

The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro MTA® (RM), EZ-Sealâ„¢ (EZ) and ENDOCEM Zr® (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ (p = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.

Keyword

Cytotoxicity; Calcium silicate; Mineral trioxide aggregate; Deciduous tooth; Stem cells
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