Nat Prod Sci.  2018 Dec;24(4):259-265. 10.20307/nps.2018.24.4.259.

Isolation of Flavonoid Glycosides with Cholinesterase Inhibition Activity and Quantification from Stachys japonica

• Nugroho A
• Choi JS
• Seong SH
• Song BM
• Park KS
• Park HJ

• KMID: 2432421
• DOI: http://doi.org/10.20307/nps.2018.24.4.259

Abstract

The three flavone glycosides, 4"²-O-methylisoscutellarein 7-O-(6"´-O-acetyl)-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (1), isoscutellarein 7-O-(6"´-O-acetyl)-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (3), and isoscutellarein 7-O-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (4) in addition to a flavonol glycoside, kaempferol 3-O-β-D-glucopyranoside (astragalin, 2), were isolated from Stachys japonica (Lamiaceae). In cholinesterase inhibition assay, compound 1 significantly inhibited aceylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities (ICâ‚…â‚€s, 39.94 µg/ml for AChE and 86.98 µg/ml for BChE). The content of isolated compounds were evaluated in this plant extract by HPLC analysis. Our experimental results suggest that the flavonoid glycosides of S. japonica could prevent the memory impairment of Alzheimer's disease.

Keyword

Stachys japonica; Lamiaceae; isoscutellarein glycoside; cholinesterase; Alzheimer's disease

MeSH Terms

Alzheimer Disease
Butyrylcholinesterase
Cholinesterases*
Chromatography, High Pressure Liquid
Glycosides*
Lamiaceae
Memory
Plants
Stachys*
Butyrylcholinesterase
Cholinesterases
Glycosides

Figure

• 

  Fig. 1. Structure of flavonoid glycosides (1–4) isolated from S. japonica and their aglycones (2a and 3a).

• 

  Fig. 2. HPLC chromatogram of standard compounds and 80% MeOH fraction of S. japonica.

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