Nat Prod Sci.  2018 Dec;24(4):259-265. 10.20307/nps.2018.24.4.259.

Isolation of Flavonoid Glycosides with Cholinesterase Inhibition Activity and Quantification from Stachys japonica

  • 1Department of Agro-industrial Technology, Lambung Mangkurat University, Banjarbaru 70714, Indonesia.
  • 2Department of Food Nutrition, Pukyong National University, Busan 48513, Korea.
  • 3Deparment of Forest Science, Sangji University, Wonju 26339, Korea.
  • 4Deparment of Oriental Medicine, Sangji University, Wonju 26339, Korea.
  • 5Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea.


The three flavone glycosides, 4"²-O-methylisoscutellarein 7-O-(6"´-O-acetyl)-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (1), isoscutellarein 7-O-(6"´-O-acetyl)-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (3), and isoscutellarein 7-O-β-D-allopyranosyl(1→2)-β-D-glucopyranoside (4) in addition to a flavonol glycoside, kaempferol 3-O-β-D-glucopyranoside (astragalin, 2), were isolated from Stachys japonica (Lamiaceae). In cholinesterase inhibition assay, compound 1 significantly inhibited aceylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities (ICâ‚…â‚€s, 39.94 µg/ml for AChE and 86.98 µg/ml for BChE). The content of isolated compounds were evaluated in this plant extract by HPLC analysis. Our experimental results suggest that the flavonoid glycosides of S. japonica could prevent the memory impairment of Alzheimer's disease.


Stachys japonica; Lamiaceae; isoscutellarein glycoside; cholinesterase; Alzheimer's disease
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