Nat Prod Sci.  2016 Mar;22(1):41-45. 10.20307/nps.2016.22.1.41.

Flavonoid Glycosides from the Flowers of Pulsatilla koreana Nakai

• Seo KH
• Jung JW
• Thi NN
• Lee YH
• Baek NI

• KMID: 2312920
• DOI: http://doi.org/10.20307/nps.2016.22.1.41

Abstract

Extraction and fractionation of Pulsatilla koreana flowers followed by, repeated open column chromatography for EtOAc and n-BuOH fractions yielded four flavonoid glycosides, namely, astragalin (1), tiliroside (2), buddlenoide A (3), and apigenin-7-O-(3"-E-p-coumaroyl)-glucopyranoside (4). The chemical structures of these flavonoid glycosides were elucidated on the basis of various spectroscopic methods including electronic ionization mass spectrometry (EI-MS), 1D NMR (1H, 13C, DEPT), 2D NMR (gCOSY, gHSQC, gHMBC), and infrared (IR) spectrometry. This study represents the first report of the isolation of the flavonoid glycosides from the flowers of P. koreana.

Keyword

Buddlenoide A; Flower; Pulsatilla koreana; Nuclear magnetic resonance; Tiliroside

MeSH Terms

Chromatography
Flowers*
Glycosides*
Magnetic Resonance Spectroscopy
Mass Spectrometry
Pulsatilla*
Spectrum Analysis
Glycosides

Figure

• 

  Fig. 1. Chemical structures of compounds 1 − 4 from the flowers of Pulsatilla koreana. OGlc: O-β-D-glucopyranosyl; Glc: β-D-glucopyranosyl; coum: p-coumaroyl.

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