Lab Anim Res.  2018 Jun;34(2):75-79. 10.5625/lar.2018.34.2.75.

Anti-Helicobacter pylori activity and inhibition of gastritis by Allium hookeri extract

• Lee HA
• Hong S
• Yoo JH
• Chung Y
• Kim O

• KMID: 2413846
• DOI: http://doi.org/10.5625/lar.2018.34.2.75

Abstract

Allium hookeri is widely consumed plant as a vegetable and herbal medicine in southeastern Asia. Allium hookeri has been reported antioxidant, improvement of bone health and antidiabetic effects. In the present study, we investigated the potential inhibitory effect of Allium hookeri extract (AHE) on Helicobacter pylori. The in vitro anti-bacterial activities of AHE were determined by disk agar diffusion method. Also, the inhibition effect of the AHE on H. pylori infection was investigated using a mouse model. H. pylori colonization was confirmed by rapid urease tests, as described previously. Mucosal damage was evaluated grossly and histologically according to previously described criteria. As the results of the disk agar diffusion assay, CLR, AMX and MTZ inhibited the bacterial growth with inhibition zone of 19.2, 15.2 and 7.5 mm, respectively. AHE 100 µg/mL showed an inhibition zone value of 20.6 mm. Rapid urease tests of the mice stomachs demonstrated a significant reduction in H. pylori colonization. In addition to the therapeutic effect against H. pylori infection, the AHE reduced mucosal inflammation and epithelial damages in the stomach of H. pylori-infected mice. These results demonstrate that the AHE successfully cured an H. pylori infection and treated the H. pylori infection. This AHE could be a promising treatment for patients with gastric complaints including gastritis caused by H. pylori.

Keyword

Allium hookeri; Helicobacter; H. pylori; gastritis; inhibition

MeSH Terms

Agar
Allium*
Animals
Asia, Southeastern
Colon
Diffusion
Gastritis*
Helicobacter
Helicobacter pylori
Herbal Medicine
Humans
In Vitro Techniques
Inflammation
Methods
Mice
Plants
Stomach
Urease
Vegetables
Agar
Urease

Figure

• 

  Figure 1 Gross findings of rapid urease test with CLO test kit. (A) Negative reaction (yellow color change) with control mice. (B) Positive reaction (red color change) with H. pylori infected mice.

• 

  Figure 2 Score of rapid urease test with CLO test kit in H. pylori infected mice was significantly decreased by Allium hookeri extracts (AHE) (*P<0.05, **P<0.01).

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