Ann Dermatol.  2017 Apr;29(2):194-199. 10.5021/ad.2017.29.2.194.

Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid

• Yoo K
• Suh KY
• Choi GH
• Kwak IS
• Seo DK
• Kym D
• Yoon H
• Cho YS
• Kim HO

• KMID: 2394843
• DOI: http://doi.org/10.5021/ad.2017.29.2.194

Abstract

BACKGROUND
It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients.
OBJECTIVE
This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters.
METHODS
Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the second, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to determine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period.
RESULTS
The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concentration of HSP70 on the first day and the length of the treatment period. No measurable concentration of IL-8 was detected before 5 hours, but the concentration started to increase after 11 hours. The peak value was measured on the fourth day.
CONCLUSION
While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of inflammatory mediators in burn blister fluid.

Keyword

Heat-shock protein 70; Interleukin-8; Partial thickness burn; Partial thickness burn blister; Wounds and injuries

MeSH Terms

Blister*
Burns*
Heat-Shock Proteins*
Hot Temperature*
HSP70 Heat-Shock Proteins*
Humans
Interleukin-8
Wound Healing
Wounds and Injuries
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
Interleukin-8

Figure

• 

  Fig. 1 (A) The heat shock protein (HSP) concentration variation within partial thickness burn blister. The HSP70 concentration was the highest on the first day (up to 12 hours after the damage). Then, the value drastically dropped down to near zero on the second day. There was a small increased on the third day and no significant amount was found on the fourth and fifth day. (B) The concentration of HSP70 within partial thickness burn blister of up to 12 hours after damage. The HSP70 concentration after one hour of the damage was measured to be the highest. After one hour, the concentration drops rapidly.

• 

  Fig. 2 (A) Variation of the interleukin-8 (IL-8) concentration within partial thickness burn blister over the time. The IL-8 concentration was measured to be the highest on the fourth day. (B) The IL-8 concentration within partial thickness burn blister up to 12 hours after damage. IL-8 was not observed up to 5 hours of the occurrence.

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