Ann Lab Med.  2017 Jan;37(1):81-83. 10.3343/alm.2017.37.1.81.

Importance of Specimen Type and Quality in Diagnosing Middle East Respiratory Syndrome

• Huh HJ
• Ko JH
• Kim YE
• Park CH
• Hong G
• Choi R
• Yu S
• Cho SY
• Kang JM
• Lee MK
• Ki CS
• Kang ES
• Lee NY
• Kim JW
• Kim YJ
• Ha YE
• Kang CI
• Chung DR
• Peck KR
• Song JH

• KMID: 2373621
• DOI: http://doi.org/10.3343/alm.2017.37.1.81

Abstract

No abstract available.

MeSH Terms

Coronavirus Infections/*diagnosis/virology
Humans
Middle East Respiratory Syndrome Coronavirus/genetics/isolation & purification
Nasopharynx/virology
RNA, Viral/genetics/metabolism
Real-Time Polymerase Chain Reaction
Viral Envelope Proteins/genetics
RNA, Viral
Viral Envelope Proteins

Figure

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  Fig. 1 Schematic representation of virus detection in respiratory tract specimens for 16 confirmed cases with negative or inconclusive results on initial testing. Positive or inconclusive results (closed); negative results (open). A dark ringed circle represents the peak cycle threshold (Ct) value.

Reference

1. Centers for Disease Control and Prevention. Novel coronavirus 2012 real-time RT-PCR assay. Updated on Jun 2014. http://www.fda.gov/downloads/MedicalDevices/Safety/EmergencySituations/UCM355572.pdf.

2. World Health Organization. Laboratory testing for Middle East Respiratory Syndrome Coronavirus. Updated on Jun 2015. http://www.who.int/csr/disease/coronavirus_infections/mers-laboratory-testing/en/.

3. Ki CS, Lee H, Sung H, Kim S, Seong MW, Yong D, et al. Korean Society for Laboratory Medicine Practice Guidelines for the Molecular Diagnosis of Middle East Respiratory Syndrome During an Outbreak in Korea in 2015. Ann Lab Med. 2016; 36:203–208. PMID: 26915607.

4. Korea Ministry of Health and Welfare (MoH) and Center for Disease Control and Prevention. Guidelins for MERS-CoV control. 3-3 ed. Updated on Jun 2015. http://www. mers.go.kr/mers/html/jsp/main.jsp.

5. Memish ZA, Al-Tawfiq JA, Makhdoom HQ, Assiri A, Alhakeem RF, Albarrak A, et al. Respiratory tract samples, viral load, and genome fraction yield in patients with Middle East respiratory syndrome. J Infect Dis. 2014; 210:1590–1594. PMID: 24837403.

6. Assiri A, Al-Tawfiq JA, Al-Rabeeah AA, Al-Rabiah FA, Al-Hajjar S, Al-Barrak A, et al. Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study. Lancet Infect Dis. 2013; 13:752–761. PMID: 23891402.

7. Drosten C, Muth D, Corman VM, Hussain R, Al Masri M, HajOmar W, et al. An observational, laboratory-based study of outbreaks of middle East respiratory syndrome coronavirus in Jeddah and Riyadh, kingdom of Saudi Arabia, 2014. Clin Infect Dis. 2015; 60:369–377. PMID: 25323704.

8. Kapoor M, Pringle K, Kumar A, Dearth S, Liu L, Lovchik J, et al. Clinical and laboratory findings of the first imported case of Middle East respiratory syndrome coronavirus to the United States. Clin Infect Dis. 2014; 59:1511–1518. PMID: 25100864.

9. MERS Joint Public and Private Sector Response Committee (MERS Joint Committee). Laborator practice guidelines. Updated on Jun 2015. http://www.mers.go.kr/mers/html/jsp/main.jsp.

10. Centers for Disease Control and Prevention. Interim guidelines for collecting, handling, and testing clinical specimens from patients under investigation (PUIs) for Middle East Respiratory Syndrome Coronavirus (MERS-CoV). V2.1. Updated on Jun 2015. http://www.cdc.gov/coronavirus/mers/guidelines-clinical-specimens.html.