J Bacteriol Virol.  2017 Sep;47(3):156-164. 10.4167/jbv.2017.47.3.156.

Study on Causes of Respiratory Disease to the Middle East Respiratory Syndrome-negative Subjects

• Hwang SJ
• Park DJ
• Koo HS
• Yun HC
• Gu PT
• Lee MO
• Jin SH

• KMID: 2392964
• DOI: http://doi.org/10.4167/jbv.2017.47.3.156

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe cases of human respiratory disease. The current outbreak of infection with this virus in South Korea, which began on May 20, 2015, has infected 186 patients and caused 36 deaths within 2 months. In this study, to investigate the viral pathogen causing acute respiratory infections, multiplex/RT-PCR was performed on were obtained from nucleic acid of the Middle East Respiratory Syndrome-negative subjects. Viruses and atypical bacteria were detected in 39 of 337 (11.6%). Frequent viruses were human rhinovirus (n=11, 3.3%), human metapneumovirus (n=9, 2.7%), parainfluenza (n=9, 2.7%) and adenovirus (n=4, 1.2%). Mycoplasma pneumonia (M. pneumonia) was detected in 1.8 % (n=6). Out of 9 human metapneumovirus (hMPV) positive samples, 6 samples were successfully sequenced using F gene. And M. pneumoniae was sequencing of a repetitive region of the P1 gene. Phylogenetic analysis revealed that hMPV clustered into A2b lineage (n=4), B2 lineage (n=2) and M. pneumoniae clustered into two genotypes: Type 1 (n=4), Type 2a (n=2).

Keyword

Middle East respiratory syndrome coronavirus (MERS-CoV); Human Metapneumovirus (hMPV); Mycoplasma pneumonia (M. pneumonia); Phylogenetic analysis

MeSH Terms

Adenoviridae
Bacteria
Genotype
Humans
Korea
Metapneumovirus
Middle East Respiratory Syndrome Coronavirus
Middle East*
Paramyxoviridae Infections
Pneumonia
Pneumonia, Mycoplasma
Repetitive Sequences, Nucleic Acid
Respiratory Tract Infections
Rhinovirus

Figure

• 

  Figure 1 Age and sex distribution of respiratory pathogens. Percentage of positive samples for each age group: 19~29 y, 3.5%; 30~39 y, 14.0%; 40~49 y, 14.3%, 50~59 y, 25.0%; ≥60 y, 9.2%.

• 

  Figure 2 Phylogenetic analysis for HMPV based on of the F protein. The nucleotide sequences were aligned using Clustal W. Trees using the neighbor-joining method with Mega 4. The scale bars show the proportions of nucleotide substitutions, and the numbers at the branches are bootstrap values determined for 1,000 iterations. Only bootstrap values with ≥70% significance are shown.

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