Ann Lab Med.  2016 Mar;36(2):185-187. 10.3343/alm.2016.36.2.185.

B-cell Acute Lymphoblastic Leukemia With t(9;22)(q34;q11) Translocation and Clonal Divergence Through ider(22) Chromosome and t(13;17)(q14;q25) Translocation

• Meza-Espinoza JP
• Romo Martinez EJ
• Aguilar Lopez L
• Picos Cardenas VJ
• Magana Torres MT
• Gonzalez Garcia JR

• KMID: 2373523
• DOI: http://doi.org/10.3343/alm.2016.36.2.185

Abstract

No abstract available.

MeSH Terms

Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 22
Chromosomes, Human, Pair 9
Female
Fusion Proteins, bcr-abl/genetics
Humans
In Situ Hybridization, Fluorescence
Karyotype
Middle Aged
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/*genetics/pathology
Retinoblastoma Binding Proteins/genetics
*Translocation, Genetic
Ubiquitin-Protein Ligases/genetics
Fusion Proteins, bcr-abl
Retinoblastoma Binding Proteins
Ubiquitin-Protein Ligases

Figure

• 

  Fig. 1 Chromosomes banded with standard techniques. (A) Metaphasic cell with the ider(22). (B) This metaphasic cell contains both t(9;22)(q34;q11.2) and t(13;17)(q14;q25). (C) In addition to the two translocations observed in the above image, a dic(1;1) chromosome is shown whose breakpoints could not be ascertained. In this picture, a single-cell chromosomal translocation t(X;15) is seen; however, it was not included in the karyotype formula based on the International System for Human Cytogenetic Nomenclature (2013) criteria [2]. Selected chromosomes: (D) 22 and ider(22); and, (E) 1 plus dic(1;1). (F) Selected C--banded by barium hydroxide and Giemsa (CBG) chromosomes 1 and dic(1;1). Note that the heterochromatic regions on the dic(1;1) chromosome apparently have different sizes, the bigger heterochromatic region being more similar to the one present in the normal chromosome 1 (×100 for A, B, and C).

• 

  Fig. 2 FISH analysis with the RB1 probe (red signals) in cells counterstained with DAPI (4',6-diamidino-2-phenylindole; blue color). (A) Metaphase from the clone with ider(22) showing two RB1 signals on both normal 13 chromosomes. (B) Cell with t(13;17) displaying only one RB1-positive signal on the normal chromosome 13; asterisks show four G group chromosomes one of which is der(13). (C) Cell with t(13;17) plus dic(1;1) also shows only one RB1-positive signal on the normal chromosome 13 (×100).

Reference

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