Cancer Res Treat.  2016 Jul;48(3):948-954. 10.4143/crt.2015.360.

Polymorphic Variants in Oxidative Stress Genes and Acute Toxicity in Breast Cancer Patients Receiving Radiotherapy

• Córdoba E
• Abba MC
• Lacunza E
• Fernánde
• Güerci AM

• KMID: 2344067
• DOI: http://doi.org/10.4143/crt.2015.360

Abstract

PURPOSE
Reactive oxygen species (ROS) are generated as an indirect product of radiation therapy (RT). Genetic variation in genes related to ROS metabolism may influence the level of RT-induced adverse effects. We evaluated the potential association of single nucleotide polymorphism (SNP)-related response to radiotherapy injury in breast cancer patients undergoing RT.
MATERIALS AND METHODS
Eighty patients receiving conventional RT were included. Acute effects were evaluated according to the Radiation Therapy Oncology Group (RTOG) scores. DNA was extracted from blood and buccal swab samples. SNPs were genotyped for GSTP1, GSTA1, SOD2, and NOS3 genes by polymerase chain reaction-based restriction fragment length polymorphism. Univariate analysis (odds ratios [ORs] and 95% confidence interval [CI]) and principal component analysis were used for correlation of SNPs and factors related to risk of developing ≥ grade 2 acute effects.
RESULTS
Sixty-five patients (81.2%) showed side effects, 32 (40%) presented moderate to severe acute skin toxicity, and 33 (41.2%) manifested minimal acute skin reactions by the end of treatment. In both univariate and multivariate analyses, nominally significant associations were found among body mass index (OR, 3.14; 95% CI, 8.5338 to 1.1274; p=0.022), breast size (OR, 5.11; 95% CI, 17.04 to 1.54; p=0.004), and grade ≥ 2 acute radiation skin toxicity. A significant association was also observed between NOS3 G894T polymorphism (OR, 9.8; 95% CI, 211.6 to 0.45; p=0.041) and grade ≥ 2 acute radiation skin toxicity in patients with neo-adjuvant chemotherapy treatment.
CONCLUSION
The analysis of the factors involved in individual radiosensitivity contributed to the understanding of the mechanisms underlying this trait.

Keyword

Breast neoplasm; Radiotherapy; Radiation tolerance; Acute toxicity; Oxidative stress; Single nucleotide polymorphism

MeSH Terms

Body Mass Index
Breast Neoplasms*
Breast*
DNA
Drug Therapy
Genetic Variation
Humans
Metabolism
Multivariate Analysis
Oxidative Stress*
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Principal Component Analysis
Radiation Tolerance
Radiotherapy*
Reactive Oxygen Species
Skin
DNA
Reactive Oxygen Species

Figure

• 

  Fig. 1. Distribution of each allele for studied genes. Obs, observed; Exp, expected; AMR, American.

Reference

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