J Korean Neuropsychiatr Assoc.  1999 May;38(3):622-629.

Developmental Changes in the Phosphorylation of CREB Following Electroconvulsive Shock in Rat Brain

Affiliations
  • 1Department of Psychiatry, Seoul National University College of Medicine, Seoul, Korea.
  • 2Department of Biochemistry, Seoul National University College of Medicine, Seoul, Korea.
  • 3Department of Biochemistry & Molecular Biology, SungKyunKwan University College of Medicine, Suwon, Korea.
  • 4Department of Neuropsychiatry, Gil Medical Center, Gachon Medical College, Incheon, Korea.

Abstract

OBJECTIVES: In order to understand the biological basis of neurodevelopmental perspectives of mental disorders, the authors investigated the developmental and regional changes in the phosphorylation of the transcription factor CREB following the electroconvulsive shock(ECS) in rat brain.
METHODS
Rats of various age groups (7, 14, 21 days postnatal and adults) were given ECS and their hippocampi and cerebella were dissected at specified time points. The content of CREB and phosphorylated CREB were measured by immunoblot analysis.
RESULTS
The amount of CREB increased in the hippocampus and decreased in the cerebellum according to the age. Baseline levels of CREB phosphorylation in both tissues were increased from postnatal 14 days, and it was proportional to the amount of CREB protein in the cerebellum. In the hippocampus, ECS increased the phosphorylation of CREB at postnatal 21 days, but in the cerebellum, ECS did not increased the phosphorylation of CREB in any age group.
CONCLUSION
CREB mediated signal transduction pathways showed developmental and tissue-specific changes. ECS increased the phosphorylation of CREB in the hippocampus by postnatal 21 days, but not in the cerebellum. CREB activation is supposed to be related with the inducdion of c-fos after ECS in the hippocampus. However, the Ser-133 phosphorylation of CREB could not completely explain the developmental and tissue specificity of c-fos induction.

Keyword

Development; Electroconvulsive shock; Rat brain; CREB; C-fos
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