J Korean Ophthalmol Soc.  1999 Jan;40(1):154-160.

The Mechanism of Tissue Injury in the Animal Model of Experimental Retinal Vein Occlusion

• Park SJ
• Chung H

Abstract

We investigated the development of programmed cell death of apoptosis as a mechanism of cell death in experimental retinal vein occlusion of rats. We induced hemicentral retinal veinocclusion by photocoagulation 3 or 4 out of 6 retinal veins after infecting rose bengal intravenously.The eyes were enucleated sequentially between day one to day fourteen, and the retinas were examined after H&E and TUNEL staining. Exudative retinal detachment developed one day after vein occlusion and was reabsorbed completely by day 4. The number of ganglion cells in vein-occluded retina began to decrease one day after vein occlusion and continue to decrease until day fourteen. Many cells in ganglion cell layer and inner nuclear layer were stained positively by TUNEL staining. These results suggest that apoptosis is an important mechanism of cell death in early stage of experimental retinal vein occlusion.

Keyword

Retinal vein occlusion; Ischemia; Apoptosis; TUNEL stain

MeSH Terms

Animals*
Apoptosis
Cell Death
Ganglion Cysts
In Situ Nick-End Labeling
Ischemia
Light Coagulation
Models, Animal*
Rats
Retina
Retinal Detachment
Retinal Vein Occlusion*
Retinal Vein*
Retinaldehyde*
Rose Bengal
Veins
Retinaldehyde
Rose Bengal