J Korean Assoc Oral Maxillofac Surg.
2010 Oct;36(5):366-374.
The effect of silk fibroin and rhBMP-2 on bone regeneration in rat calvarial defect model
- Affiliations
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- 1Department of Oral and Maxillofacial Surgery, Kangnam Sacred Heart Hospital, College of Medicine, Korea. kilhyun73@naver.com
- 2Department of Oral and Maxillofacial Implantology, Clinical Graduate School of Dentistry, Hallym University, Korea.
- 3National Academy of Agricultural Science, Rural Development Administration, Korea.
- 4Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Korea.
Abstract
This study evaluated the capability of silk fibroin (SF) and recombinant human bone morphogenetic protein-2 loaded SF (SF-BMP) as a bone defect replacement matrix when grafted in a calvarial bone defect of rats in vivo.
MATERIALS AND METHODS
A total 70 calvarial critical size defects (5.0 mm in diameter) made on 35 adult female Sprague-Dawley rats were used in this study. The defects were transplanted with (1) rhBMP-2 loaded silk fibroin graft (SF-BMP: 0.8+10 microg), (2) Silk fibroin (SF: 10 microg), and (3) no graft material (Raw). The samples were evaluated with soft x-rays, alkaline phosphatase activity, calcium/phosphate quantification, histological and histomorphometric analysis at postoperative 4 and 8 weeks.
RESULTS
The SF-BMP group (48.86+/-14.92%) had a significantly higher mean percentage bone area than the SF group (24.96+/-11.01%) at postoperative 4 weeks.(P<0.05) In addition, the SF-BMP group (40.01+/-12.43%) had a higher % bone area at postoperative 8 weeks than the SF group (33.26+/-5.15%). The mean ratio of gray scale levels to the host bone showed that the SF-BMP group (0.67+/-0.08) had a higher mean ratio level than the SF group (0.61+/-0.09) at postoperative 8 weeks. These differences were not statistically significant.(P=0.168 and P=0.243, respectively)
CONCLUSION
The rhBMP-2 loaded silk fibroin graft revealed fewer immunoreactions and inflammation as well as more new bone formation than the pure silk fibroin graft. Therefore, silk fibroin may be a candidate scaffold for tissue engineered bone regeneration.
Keyword
MeSH Terms
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Adult
Alkaline Phosphatase
Animals
Bone Morphogenetic Protein 2
Bone Regeneration
Female
Fibroins
Humans
Inflammation
Osteogenesis
Rats
Rats, Sprague-Dawley
Recombinant Proteins
Silk
Tissue Scaffolds
Transforming Growth Factor beta
Transplants
Alkaline Phosphatase
Bone Morphogenetic Protein 2
Fibroins
Recombinant Proteins
Silk
Transforming Growth Factor beta
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