J Korean Assoc Oral Maxillofac Surg.  2010 Oct;36(5):341-345.

Evaluation of osteogenic activity of periosteal-derived cells treated with inflammatory cytokines

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea. surbyun@gsnu.ac.kr
  • 2Biomedical center, Brain Korea 21 (BK21), Gyeongsang National University, Jinju, Korea.
  • 3Clinical Research Institute, Gyeongsang National University Hospital, Jinju, Korea.
  • 4Department of Biochemistry, School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Korea.
  • 5Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea.
  • 6Maxillofacial Center, Onhospital, Busan, Korea.

Abstract

INTRODUCTION
Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells.
MATERIALS AND METHODS
Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco's modified Eagle's medium (DMEM) medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-alpha with different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1beta with different concentrations (0.01, 0.1, and 1 ng/mL) were added.
RESULTS
Both TNF-alpha and IL-1beta stimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-alpha and IL-1beta increased the level of ALP expression in a dose-dependent manner. Both TNF-alpha and IL-1beta also increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells.
CONCLUSION
These results suggest that inflammatory cytokines TNF-alpha and IL-1beta can stimulate the osteoblastic activity of cultured human periosteal-derived cells.

Keyword

Periosteal-derived cells; Inflammatory cytokine; Tumor necrosis factor-alpha (TNF-alpha); Interleukin-1beta (IL-1beta)

MeSH Terms

Alkaline Phosphatase
Anthraquinones
Ascorbic Acid
Bone Resorption
Cell Culture Techniques
Cytokines
Dexamethasone
Durapatite
Glycerophosphates
Homeostasis
Humans
Interleukins
Osteoblasts
Osteoclasts
Osteogenesis
Periosteum
Tumor Necrosis Factor-alpha
Alkaline Phosphatase
Anthraquinones
Ascorbic Acid
Cytokines
Dexamethasone
Durapatite
Glycerophosphates
Interleukins
Tumor Necrosis Factor-alpha
Full Text Links
  • JKAOMS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr