J Korean Assoc Maxillofac Plast Reconstr Surg.  2010 May;32(3):199-206.

STIMULATION OF OSTEOBLASTIC PHENOTYPES BY STRONTIUM IN PERIOSTEAL-DERIVED CELLS

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, Korea.
  • 2Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical center (BK21), Jinju, Korea. surbyun@gsnu.ac.kr
  • 3Clinical Research Institute, Gyeongsang National University Hospital, Jinju, Korea.
  • 4Department of Biochemistry, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical center (BK21), Jinju, Korea.
  • 5Department of Oral and Maxillofacial Surgery, Onhospital, Yangsan, Korea.

Abstract

This study investigated the effects of strontium on osteoblastic phenotypes of cultured human periosteal-derived cells. Periosteal tissues were harvested from mandible during surgical extraction of lower impacted third molar. Periosteal-derived cells were introduced into cell culture. After passage 3, the periosteal-derived cells were further cultured for 28 days in an osteogenic induction DMEM medium supplemented with fetal bovine serum, ascorbic acid 2-phosphate, dexamethasone and at a density of 3 x 104 cells/well in a 6-well plate. In this culture medium, strontium at different concentrations (1, 5, 10, and 100 microg/mL) was added. The medium was changed every 3 days during the incubation period. We examined the cellular proliferation, histochemical detection and biochemical measurements of alkaline phosphatase (ALP), the RT-PCR analysis for ALP and osteocalcin, and von Kossa staining and calcium contents in the periosteal-derived cells. Cell proliferation was not associated with the addition of strontium in periosteal-derived cells. The ALP activity in the periosteal-derived cells was higher in 5, 10, and 100 microg/ml strontium-treated cells than in untreated cells at day 14 of culture. Among the strontium-treated cells, the ALP activity was appreciably higher in 100 microg/ml strontium-treated cells than in 5 and 10 microg/ml strontium-treated cells. The levels of ALP and osteocalcin mRNA in the periosteal-derived cells was also higher in strontium-treated cells than in untreated cells at day 14 of culture. Their levels were increased in a dose-dependent manner. Von Kossa-positive mineralization nodules were strongly observed in the 1 microg/ml strontium-treated cells at day 21 and 28 of culture. The calcium content in the periosteal-derived cells was also higher in 1 microg/ml strontium-treated cells at day 28 of culture. These results suggest that low concentration of strontium stimulates the osteoblastic phenotypes of more differentiated periosteal-derived cells, whereas high concentration of strontium stimulates the osteoblastic phenotypes of less differentiated periosteal-derived cells. The effects of strontium on osteoblastic phenotypes of periosteal-derived cells appear to be associated with differentiation-extent.

Keyword

Periosteal-derived cells; Strontium; Osteoblastic phenotypes

MeSH Terms

Alkaline Phosphatase
Ascorbic Acid
Calcium
Cell Culture Techniques
Cell Proliferation
Dexamethasone
Durapatite
Humans
Mandible
Molar, Third
Osteoblasts
Osteocalcin
Phenotype
RNA, Messenger
Strontium
Alkaline Phosphatase
Ascorbic Acid
Calcium
Dexamethasone
Durapatite
Osteocalcin
RNA, Messenger
Strontium
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