Tuberc Respir Dis.  2013 Nov;75(5):205-209.

Effect of Prunetin on TNF-alpha-Induced MUC5AC Mucin Gene Expression, Production, Degradation of IkappaB and Translocation of NF-kappaB p65 in Human Airway Epithelial Cells

Affiliations
  • 1Department of Pharmacology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea. LCJ123@cnu.ac.kr
  • 2Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Korea.

Abstract

BACKGROUND
We investigated whether prunetin significantly affects tumor necrosis factor-alpha (TNF-alpha)-induced MUC5AC mucin gene expression, production, inhibitory kappa B (IkappaB) degradation and nuclear factor kappa B (NF-kappaB) p65 translocation in human airway epithelial cells.
METHODS
Confluent NCI-H292 cells were pretreated with prunetin for 30 minutes and then stimulated with TNF-alpha for 24 hours or the indicated periods. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The effect of prunetin on TNF-alpha-induced degradation of IkappaB and translocation of NF-kappaB p65 was investigated by western blot analysis.
RESULTS
We found that incubation of NCI-H292 cells with prunetin significantly inhibited mucin production and down-regulated the MUC5AC gene expression induced by TNF-alpha. Prunetin inhibited TNF-alpha-induced degradation of IkappaB and translocation of NF-kappaB p65.
CONCLUSION
This result suggests that prunetin inhibits the NF-kappaB signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production regulated by the NF-kappaB signaling pathway.

Keyword

Mucins; MUC5AC Protein, Human; Prunetin

MeSH Terms

Blotting, Western
Enzyme-Linked Immunosorbent Assay
Epithelial Cells*
Gene Expression*
Humans*
Isoflavones
Mucin 5AC
Mucins*
NF-kappa B*
Polymerase Chain Reaction
Reverse Transcription
Tumor Necrosis Factor-alpha
Isoflavones
Mucin 5AC
Mucins
NF-kappa B
Tumor Necrosis Factor-alpha
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