Pediatr Allergy Respir Dis.  2006 Dec;16(4):306-316.

Comparison of Cytokine and Chemokine Production and Activation of NF-kappaB in RSV- and Adenovirus-infected Bronchial Epithelial Cells

Affiliations
  • 1Department of Pediatrics, College of Medicine, the Catholic University of Korea, Seoul, Korea. hhkim@hfh.cuk.ac.kr
  • 2Catholic Research Institutes of Medical Science, the Catholic University of Korea, Seoul, Korea.

Abstract

PURPOSE
Our study was designed to evaluate the effects of respiratory syncytial virus (RSV) and adenovirus (Ad) on the production of interleukin (IL)-6, IL-8 and RANTES by BEAS-2B cells. The role of NF-kappaB in the production of those was also studied.
METHODS
We studied the concentration of IL-6, IL-8, RANTES in the culture supernatant of BEAS-2B cell infected by RSV, adenovirus type 3 (Ad3), Ad7. The activation of NF-kappaB was also investigated and the role in the production of IL-6, IL-8, RANTES compared among RSV-, Ad3-, and Ad7- infected cells by electromobility shift assay (EMSA).
RESULTS
The protein production of RANTES, IL-6, and IL-8 in RSV infection was more than Ad infection. The NF-kappaB binding activities in the promotor genes of RANTES, IL-6, IL-8 appeared in RSV, Ad3, and Ad7. These activities of NF-kappaB were inhibited by each gene specific probe. CONSULSION:We suggest that such differences in the immune responses between RSV and Ad may affect the clinical patterns and prognosis after RSV and Ad infection.

Keyword

Interleukin-6; Interleukin-8; RANTES; NF-kappaB

MeSH Terms

Adenoviridae
Chemokine CCL5
Epithelial Cells*
Interleukin-6
Interleukin-8
Interleukins
NF-kappa B*
Prognosis
Respiratory Syncytial Viruses
Chemokine CCL5
Interleukin-6
Interleukin-8
Interleukins
NF-kappa B
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