Osteoporosis.  2012 Dec;10(3):119-128.

The Association between Single Nucleotide Polymorphisms of Sclerostin (SOST) Gene, Bone Mineral Density and Circulating Osteoprotegerin (OPG) - Soluble Receptor Activator of NF-kappaB Ligand (sRANKL) in Postmenopausal Korean Women

• Lee HJ
• Kim H
• Ku SY
• Kim SH
• Choi YM
• Kim JG

Abstract

OBJECTIVES
To investigate the relationship between polymorphisms of sclerostin (SOST) gene and bone mineral density (BMD) in postmenopausal Korean women.
MATERIALS AND METHODS
The SOST rs865429 (g.5942C>T), rs17882143 (c.28G>A), rs10534024 (-1396TCC/Del) polymorphisms were analyzed by Taqman assay and direct DNA sequencing in 399 postmenopausal Korean women. Serum CrossLaps (CTX), bone alkaline phosphatase (BAP), osteocalcin, osteoprotegerin (OPG) and soluble receptor activator of NF-kB ligand (sRANKL) were measured by enzyme linked immunosorbent assay. The BMD at the lumbar spine (LS) and femoral neck (FN) were determined by dual energy X-ray absorptiometry, and in 311 postmenopausal women receiving hormone therapy (HT) BMD was also measured after HT of 1 year.
RESULTS
The SOST g.5942C>T and c.28G>A polymorphisms were not observed. No significant differences in adjusted BMD at LS and FN, prevalence for osteoporosis and serum levels of biochemical markers were noted among genotypes of the SOST -1396TCC/del polymorphism. A significant association was found between SOST -1396TCC/Del polymorphism and the annual percent changes of BMD at LS but not FN after HT. The Del/Del genotype showed a significant decrease in BMD after HT compared with other genotypes.
CONCLUSIONS
The SOST -1396TCC/Del polymorphism affects an annual BMD change at LS after HT in postmenopausal Korean women.

Keyword

BMD; Genetic polymorphism; Hormone therapy; SOST

MeSH Terms

Absorptiometry, Photon
Alkaline Phosphatase
Biomarkers
Bone Density
Collagen
Enzyme-Linked Immunosorbent Assay
Female
Femur Neck
Genotype
Humans
NF-kappa B
Osteocalcin
Osteoporosis
Osteoprotegerin
Peptide Fragments
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Prevalence
Receptor Activator of Nuclear Factor-kappa B
Sequence Analysis, DNA
Spine
Alkaline Phosphatase
Collagen
NF-kappa B
Osteocalcin
Osteoprotegerin
Peptide Fragments
Receptor Activator of Nuclear Factor-kappa B