J Korean Soc Osteoporos.  2010 Aug;8(2):178-187.

The Relationship between Wnt Signaling Pathway Gene Polymorphisms and Production of Osteoprotegerin and Soluble Receptor Activator of NF-kB by Whole Blood Cells

• Lee DY
• Kim H
• Ku SY
• Suh CS
• Kim SH
• Choi YM
• Kim JG

Abstract

OBJECTIVES
To investigate the relationship between single nucleotide polymorphisms (SNPs) in Wnt signal pathway genes, and production of osteoprotegerin (OPG) and soluble receptor activator of NF-kappaB ligand (sRANKL) by whole blood cells in postmenopausal Korean women.
METHODS
Low density lipoprotein receptor-related protein (LRP) 5 c266 A>G and c3893 C>T, frizzled (FZD) 6 c1033 A>C, axin II c148 C>T, T cell factor (Tcf) 1 c766 G>A, and adenomatous polyposis coli (APC) c5465 T>A polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), direct sequencing, and Taqman assay in 141 postmenopausal Korean women. The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells were also measured.
RESULTS
The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells, and their ratio of RANKLx1,000/OPG were not different according to the LRP 5 c266 A>G and c3893 C>T, FZD 6 c1033 A>C, axin II c148 C>T, Tcf 1 c766 G>A, and APC c5465 T>A polymorphisms. No significant differences in the production of OPG and sRANKL, and their ratio of RANKLx1,000/OPG were noted among combined genotypes of LRP c266 A>G and c3893 C>T polymorphisms.
CONCLUSION
LRP 5 c266 A>G and c3893 C>T, FZD 6 c1033 A>C, axin II c148 C>T, Tcf 1 c766 G>A, and APC c5465 T>A polymorphisms do not affect the production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells.

Keyword

Whole blood cell; Osteoprotegerin; RANKL; Polymorphism

MeSH Terms

Adenomatous Polyposis Coli
Blood Cells
Female
Genotype
Humans
Lipoproteins
NF-kappa B
Osteoprotegerin
Polymorphism, Single Nucleotide
Receptor Activator of Nuclear Factor-kappa B
Signal Transduction
Wnt Signaling Pathway
Lipoproteins
NF-kappa B
Osteoprotegerin
Receptor Activator of Nuclear Factor-kappa B