Korean J Urol.  1998 Aug;39(8):723-728.

Biochemical Analysis of the Changes of Collagen Types following Bladder Outlet Obstruction in the Rat

• Kim KM
• Jung JY
• Kim DK
• Cheong HI

Abstract

PURPOSE: We observed the ratio of collagen type III to type I by biochemical analysis in the adult rat bladder with chronic partial bladder outlet obstruction. MATERIALS AND METHODS: Female adult(20-wk-old, 200-250gm) Sprague-Dawley rats were divided into 3 groups: control group. 6-wk obstruction group, and 12-wk-obstruction group(n=7 in each group), Collagen samples, extracted from the whole bladders by pepsin digestion followed by salt precipitation, were analysed by SDS-PAGE on 5% or 8% polyacrylamide slab gels with in situ reduction or pre-reduction. Collagen polypeptides were wisualized by Coomassie blue staining and quantitated with a scanning densitometer. Collagen types were confirmed y Western blots.
RESULTS
The ratio of collagen type III to type I increased gradually alona the obstructed period and the highest value was found in 12-wk-obstruction group(sontrol 0.29+/-0.023, 6-wk-obstruction 0.332+/-0.026, and 12-wk-obstruction0.395+/-0.047, mean+/- standard error). This change was statistically significant in the 12-wk-obstruction group compared to that of control. (p<0.05; by Wilcoxon signed rank test)
CONCLUSIONS
Increasing type III collagen compared to type I collagen is a unique feature in the obstructed bladder.

Keyword

Rat; Bladder outlet obstruction; Collgen type

MeSH Terms

Adult
Animals
Blotting, Western
Collagen Type I
Collagen Type III
Collagen*
Digestion
Electrophoresis, Polyacrylamide Gel
Female
Gels
Humans
Pepsin A
Peptides
Rats*
Rats, Sprague-Dawley
Urinary Bladder Neck Obstruction*
Urinary Bladder*
Collagen
Collagen Type I
Collagen Type III
Gels
Pepsin A
Peptides