Ann Dermatol.
2013 Feb;25(1):73-79. 10.5021/ad.2013.25.1.73.
Molecular Biological Identification of Malassezia Yeasts Using Pyrosequencing
- Affiliations
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- 1Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea. 20050078@kuh.ac.kr
- 2Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
- KMID: 2265974
- DOI: http://doi.org/10.5021/ad.2013.25.1.73
Abstract
- BACKGROUND
A Pyrosequencing assay has been used in identification of fungal species such as Candida or Aspergillus and diagnosis of pathogenic bacteria such as Helicobacter pylori but there has been no report on successful isolation and identification of Malassezia yeasts using the pyrosequencing method.
OBJECTIVE
Examine the applicability and plausibility of the pyrosequencing method in identification of the Malassezia species.
METHODS
At internal transcribed spacer (ITS) sites 1 and 2, three primers were developed using Pyrosequencing Assay Design Software (Biotage AB). Pyrosequencing was performed on 11 standard strains and 83 genomic DNA samples obtained from 66 healthy controls aged from 1 to 80.
RESULTS
The eleven Malassezia standard species and 83 genomic DNA samples were successfully identified using the pyrosequencing assay.
CONCLUSION
The pyrosequencing method is a new tool for analysis of Malassezia yeasts, and its precision and rapidity suggests its clinical applicability.
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