Ann Dermatol.  2010 May;22(2):149-155. 10.5021/ad.2010.22.2.149.

Epidemiologic Study of Malassezia Yeasts in Seborrheic Dermatitis Patients by the Analysis of 26S rDNA PCR-RFLP

• Oh BH
• Lee YW
• Choe YB
• Ahn KJ

• KMID: 2265384
• DOI: http://doi.org/10.5021/ad.2010.22.2.149

Abstract

BACKGROUND
This case-control study concerns a molecular biological method based on the data gathered from a group of Korean subjects to examine the distribution of Malassezia yeasts in seborrheic dermatitis (SD) patients. Cultures for Malassezia yeasts were taken from the foreheads, cheeks and chests of 60 patients with SD and in 60 healthy controls of equivalent age.
OBJECTIVE
The purpose of this study is to identify the relationship between certain species of Malassezia and SD. This was done by analyzing the differences in the distribution of Malassezia species in terms of age and body parts of the host with healthy controls.
METHODS
26S rDNA PCR-RFLP, a fast and accurate molecular biological method, was used to overcome the limits of morphological and biochemical methods. RESULTS: The positive Malassezia culture rate was 51.7% in patients with SD, which was lower than that of healthy adults (63.9%). M. restricta was dominant in patients with SD (19.5%). Likewise, M. restricta was identified as a common species (20.5%) in healthy controls. In the ages 31~40, M. restricta was found to be the most common species (31.6%) among SD patients.
CONCLUSION
According to the results of the study, the most frequently isolated species was M. restricta (19.5%) in patients with SD. There was no statistically significant difference in the distribution of Malassezia species between the SD patients and healthy control groups.

Keyword

26S rDNA PCR-RFLP; Malassezia yeasts; Seborrheic dermatitis

MeSH Terms

Adult
Case-Control Studies
Cheek
Dermatitis, Seborrheic
DNA, Ribosomal
Epidemiologic Studies
Forehead
Human Body
Humans
Malassezia
Thorax
Yeasts
DNA, Ribosomal

Figure

• 

  Fig. 1 PCR-RFLP patterns of 26S rDNA PCR digested with Hha I (A), BtsC I (B) of 11 Malassezia standard strains. Lanes: M: molecular marker, 1: M. furfur (KCTC 7743), 2: M. sympodialis (KCTC 7985), 3: M. globosa (CBS 7966), 4: M. restricta (KCTC 7848), 5: M. slooffiae (KCTC 17431), 6: M. pachydermatis (KCTC 17008), 7: M. japonica (CBS 9432), 8: M. nana (JCM 12085), 9: M. dermatis (JCM 11348), 10: M. obtusa (KCTC 7847), 11: M. yamatoensis (CBS 9725).

• 

  Fig. 2 PCR-RFLP patterns of 26S rDNA PCR digested with Hha I (A), BtsC I (B) of Malassezia yeasts from seborrheic dermatitis patients and healthy controls. Lanes: M: molecular marker, 1: M. restricta, 2: M. globosa, 3: M. sympodialis, 4: M. furfur, 5: M. dermatis, 6: M. slooffiae (from healthy control), 7: M. obtusa (from healthy control).

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  Fig. 3 Identified Malassezia species from seborrheic dermatitis group compared with healthy control group, by ages.

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  Fig. 4 Identified Malassezia species from seborrheic dermatitis group compared with healthy control group, by body sites.

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