Korean J Hepatobiliary Pancreat Surg.
2010 Jun;14(2):87-93.
Hepatic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell Using Fibrin Gels in 70% Hepatectomized Rat
- Affiliations
-
- 1Department of Surgery, Chonnam National University College of Medicine, Chonnam, Korea.
- 2Department of Surgery, Seoul National University College of Medicine, Seoul, Korea. sejung@plaza.snu.ac.kr
- 3Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
Abstract
- PURPOSE
To investigate the differentiation of rat bone marrow-derived mesenchymal stem cells (MSCs) into hepatocytes by cell transplantation using fibrin gels in a 70% hepatectomized rat model.
METHODS
MSCs were isolated from Sprague-Dawley rats. MSCs (1.5x10(7) cells) were mixed with fibrin gels and injected immediately into the abdominal cavity of 70% hepatectomized rats. Fibrin-gels consisted of 500 IU/ml of thrombin and 90 mg/ml of fibrinogen. Transplanted MSCs in the fibrin scaffold were retrieved from surgically opened peritoneal cavities of rats on days 5, 10, 15, and 21 after the operation. The specimens were analyzed histologically and immunohistochemically.
RESULTS
On H&E staining, MSCs from hepatectomized rats had changed to a round shape, while MSCs of the control group kept their spindle shape. When the fibrin matrix was biodegraded at day 15, the morphology of the MSCs had changed to hepatocyte-like cells without sinusoids and the hepatocyte-like cells had formed a three-dimensional tissue permitting cell-to-cell contacts within the matrix. On immunohistochemistry, MSCs expressed the hepatocyte markers cytokeratin 18, albumin, and alpha-fetoprotein, after 15 days of transplantation.
CONCLUSION
When bone marrow-derived MSCs are transplanted using fibrin gels in the 70% hepatectomized rat, MSCs differentiate into hepatocyte-like cells and are conglomerated so that they form three-dimensional tissue-like hepatocytes without sinusoids.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Differential Potential of Stem Cells Following Their Origin: Subacromial Bursa, Bone Marrow, Umbilical Cord Blood
- Bone tissue engineering using PLLA/HA composite scaffold and bone marrow mesenchymal stem cell
- Clinical Safety and Efficacy of Autologous Bone Marrow-Derived Mesenchymal Stem Cell Transplantation in Sensorineural Hearing Loss Patients
- Effects of bFGF and PDGF-BB on osteoblast differentiation of bone marrow-derived mesenchymal stem cell in rat
- Concise Review: Differentiation of Human Adult Stem Cells Into Hepatocyte-like Cells In vitro
