J Korean Ophthalmol Soc.  2003 Jan;44(1):202-207.

Effects of Taxol and Mitomycin C on Morphologic Alteration of Cultured Rabbit Subconjunctival Fibroblasts

• Baek YW
• Moon JI
• Park CK

Abstract

PURPOSE
Scar tissue formation by cell proliferation, migration and contraction of fibroblasts may result in the failure of trabeculectomy. The author investigated effects of Mitomycin C (MMC) and Taxol on morphologic alteration of cultured rabbit subconjunctival fibroblasts. METHODS: Indirect immunofluorescence microscopy, with antibodies against tubulin, was used to examine the effect of Mitomycin C and Taxol on cytoplasmic microtubules in rabbit. Tenon's fibroblast cells and Acridine orange/Ethidium bromide dyes were used to determine cell viability and apoptosis at various concentrations of MMC and Taxol. RESULTS: Taxol treated cell showed an increased intensity of microtubule staining around the nuclear region and morphologic differences in overall cell shape, and MMC treated cells were noted more prominent apoptotic changes. CONCLUSIONS: Our result suggested that Taxol might be as potent inhibitor of fibroblast proliferation as Mitomycin C, and therefore, might be used for improving the success rate of trabeculectomy.

Keyword

Fibroblast; Microtubule; Mitomycin C; Taxol

MeSH Terms

Antibodies
Apoptosis
Cell Proliferation
Cell Shape
Cell Survival
Cicatrix
Coloring Agents
Cytoplasm
Fibroblasts*
Fluorescent Antibody Technique, Indirect
Microscopy
Microtubules
Mitomycin*
Paclitaxel*
Trabeculectomy
Tubulin
Antibodies
Coloring Agents
Mitomycin
Paclitaxel
Tubulin