J Korean Ophthalmol Soc.  2002 Jul;43(7):1293-1301.

Immunohistochemical Distribution of Heme Oxygenase Isoenzymes in Developing Rat Retina

• Kang YK
• Lee HY
• Ahn CS

Abstract

PURPOSE: To analyze and compare the immunohistochemical distributions of HO-1 and HO-2 with the developmental process of retina, focusing on the time of birth and opening eyes because it is the time of the exposure to hyperoxia and light exposure.
METHODS
The retina of 16-day-old embryo, 18-day-old embryo, 20-day-old embryo and postnatal rats at 0 day, 5 days, 10 days, 15 days, 20 days, 1 month, 2 months, 3 months were examined by hematoxylineeosin staining and immunohistochemistry of HO-1 and HO-2.
RESULTS
Similar distribution of HO-1 and HO-2 from embryo to mature retina were observed. It was observed that HO-1 and HO-2 was contained in ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer and pigment layer of the mature retina. In the developmental process, reactivity was observed in the cell body of ganglion cells after 0 day of its birth. The reactivity was increased after 5 days of its birth, staining was observed in nerve cell and nerve fiber of ganglion cell layer, inner plexiform layer, inner segment of neuroblastic layer, pigment layer at this time. Reactivity of ganglion cell layer and pigment layer was increased intensively after 15 days of its birth and weak reactivity in some of horizontal cells and amacrine cells in inner plexiform layer was observed. The same distribution as that of mature retina was observed after 20 days of its birth.
CONCLUSIONS
HO-1 and HO-2 expression was temporally and spatially correlated with the normal development of retina in rats. HO was concerned with the structure and function of retinal development by the activation of its products and with anti-oxidant mechanism on the time of birth and opening eyes.

Keyword

Heme oxygenase-1 and Heme oxygenase-2; Retinal development

MeSH Terms

Amacrine Cells
Animals
Embryonic Structures
Ganglion Cysts
Heme Oxygenase (Decyclizing)*
Heme*
Hyperoxia
Immunohistochemistry
Isoenzymes*
Nerve Fibers
Neurons
Parturition
Rats*
Retina*
Retinaldehyde
Heme
Heme Oxygenase (Decyclizing)
Isoenzymes
Retinaldehyde